Use [Ef] to define Vmax using the equation vmax = kcat [Et]. Consult a college level biochemistry guide or textbook for detailed instructions on how to solve each of these equations and for specific definitions of each variable. Step 4 Insert Km, [S] and Vmax into the Michaelis-Menten ...
To use the nomograph, connect two known values using a ruler and read the unknown value on the third scale. Start with one of the known values. For example, if the boiling point at sea level is known and the barometric pressure is known, connect those two points with a ruler. Extending...