If you plot enzyme velocity as a function of subtrate concentration, you can fit the data to the Michaelis-Menten equation to determine the Kmand Vmax. The Vmaxis the maximum enzyme velocity extrapolated out to very high concentrations of substrate. It is expressed in the same units you used...
Re: Enzyme Kinetics: Units of kcat? «Reply #4 on:October 08, 2009, 02:32:54 PM » Yes, Vmaxis dependent on enzyme concentration from the equation you provided: Vmax= kcat[E], where kcatis independent of the enzyme concentration. So, you are correct, if you double the enzyme conc...
Enzyme Kinetics Equation | Definition & Examples from Chapter 6/ Lesson 5 51K Learn how to measure enzyme activity. Discover the factors that affect enzyme kinetics, and work through examples of the Michaelis-Menten enzyme equation. Related to this Question ...
The deep learning model was able to show enzyme promiscuity. Understanding enzyme promiscuity and the related underground metabolism is a key topic in evolutionary biology22,23. DLKcat-predictedkcatvalues (Fig.2c) were higher for preferred substrates (mediankcat = 11.07 s–1) compared to...
kcat/KM- This ratio is often thought of as a measure of enzyme efficiency. Either a large value ofkcat(rapid turnover) or a small value ofKM(high affinity for substrate) makeskcat/KMlarge. When [S] <<KM(dilute solution), equation 11.27 becomes V (kcat/KM)[E][S] Here,kcat/KMbehave...
kcat/KM - This ratio is often thought of as a measure of enzyme efficiency. Either a large value of kcat (rapid turnover) or a small value of KM (high affinity for substrate) makes kcat/KM large. When [S] << KM (dilute solution), equation 11.27 becomes V (kcat/KM)[E][S] Here...
Following this, the CNN used a filter function, shown in equation (1), to compute the hidden vectors from the input word embeddings and weight matrix. After that, we obtained a set of hidden vectors for these split subsequences based on n-gram amino acid splitting. c(i t) = f(Wconvc...
kcat/KM- This ratio is often thought of as a measure of enzyme efficiency. Either a large value ofkcat(rapid turnover) or a small value ofKM(high affinity for substrate) makeskcat/KMlarge. When [S] <<KM(dilute solution), equation 11.27 becomes V (kcat/KM)[E][S] Here,kcat/KMbehave...