Proposes an approach to encoding that will allow genomics companies to make DNA sequences available to the public while retaining some intellectual property protection. DNA sequence back-translation; Copyright infringement.StemmerWillem P.C.Nature Biotechnology...
Nucleic Acid ConformationMolecular Sequence DataModels, MolecularX-ray crystallography can reveal the three-dimensional structure of short fragments of DNA or ... F Hays,A Teegarden,ZJR Jones,... 被引量: 4发表: 2005年 How sequence defines structure: A crystallographic map of DNA structure and co...
The field of molecular epidemiology is very promising, as sophisticated techniques are being developed to address etiology, genetic susceptibility and mechanisms for induction of disease. The use of biomarkers plays a key role in these investigations because the information can be used to predict the ...
DNA Ladders Selection Guide PCR Enzymes PCR and qPCR Plastic Consumables Reverse Transcriptase Restriction EnzymeIntroducing a universal annealing temperature for primers Primer annealing is a critical step in polymerase chain reaction or PCR. In this step, t...
We do not show asORFs in frames 0 and 2 because their probabilities are identical to that of igORFs. The probability of asORFs relative to that of igORFs (\({\log }_{2}\) ratio, vertical axis), calculated using frequencies of short DNA sequences from C the yeast genome, and D ...
Cloned DNA (cDNA) libraries are produced from the mRNA present in a cell. Complementary DNA sequences can be made and inserted into new cells. These fragments are typically spliced into a plasmid and inserted into a live cell. Knowing the sequence of the plasmid is very helpful in studying ...
sequencing by CE is used to determine the specific base sequence of a particular fragment or gene segment, this technique can also provide sizing, relative quantitation, and genotyping information for fluorescently labeled DNA fragments produced by PCR using primers des...
It uses the sequence of the sister chromatid as a template. First, the MRN complex (Mre11, Rad50, and Nbs1) binds to one side of the break. MRN and Sae2 next create short 3’ overhangs by chopping the 5’ ends around the DSB. RPA and Rad51 coat the single strands and form a ...
Provides a web site related to sequence-tagged sites (STS) within a DNA. Information on the STS markers within a DNA fragment; Details of the genomic context of the STS marker in all chromosomal maps.doi:10.1038/ng967NoneNature Genetics...
For DNA microarray spotting, the individual DNA sequences need to be chemically synthesized or produced via cell culture in advance. However, when choosing chemical synthesis, the DNA length is limited to short oligomers because of increasing synthesis errors. Hence, for longer DNA sequences, cell...