HID lamps use less power and are much brighter than most fluorescent and incandescent lamps. These are typically used when high levels of light over large areas are required, and when energy efficiency and light intensity are desired. HID bulbs are more fragile, have to warm up and are suscep...
The resulting rolling circular amplification (RCA) serves as a target for hybridized fluorescently labeled detection oligonucleotides allowing distinct and bright spots to be identified and quantified in a fluorescent microscope. See http://www.olink.com/movie.php for a graphic movie of this technology...