These survey results highlight that member companies agree that applying a one-size-fits-all approach to the execution of stability studies for CGT products is not feasible. Alternate solutions are required to minimize the impact on batch yield; however, specific recommendations vary based ...
run on an sds-page gel and stained with a colloidal coomassie stain. the same gel was imaged 3 ways. figure 1. coomassie-stained gel imaged on the azure 300 imager on a white light transilluminator learn more about the azure 300 figure 2. coomassie-stained gel imaged on chemisolo using w...
What is SDS PAGE gel electrophoresis? What factors affect gel electrophoresis? What could be achieved by DNA profiling using gel electrophoresis? What are the applications of gel electrophoresis? What is pulsed field gel electrophoresis? What is the purpose and general process of gel electrophoresis?
Transcriptional bursting has been linked to the stochastic positioning of nucleosomes. However, how bursting is regulated by the remodeling of promoter nucleosomes is unknown. Here, we use single-molecule live-cell imaging of GAL10 transcription in Sacch
Similarly to the report by Ulinski et al., sodium dodecyl sulfate polyacrylamide gel electrophoresis ( SDS-PAGE ) of our patient's urine detected only albumin fraction without transferrin band (Fig. 1 , lane 2) although total protein excretion was significant (2.8g/l). Such electrophoregraphic...
GDP-mannose (GDP-Man) is a key metabolite essential for protein glycosylation and glycophosphatidylinositol anchor synthesis, and aberrant cellular GDP-Man levels have been associated with multiple human diseases. How cells maintain homeostasis of GDP-Ma
This allows to resolve proteins of very similar mass, notably unmodified and small molecular mass post-translationnally modified (e.g. phosphorylated) variants of a same protein. IEF is often used upstream of SDS-PAGE in so-called 2D gel electrophoresis [21] . Capillary electrophoresis (CE) is...
Analysis: The absorbance at 280 nm was measured for all protein fractions to determine the amount of total protein. Samples were separated via SDS-PAGE and stained with Coomassie® blue and the enzyme activity was measured using a Malachite green phosphate assay. Results and discussion The ...
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
All reactions were carried out at 37 °C for 10 min, stopped by addition of EDTA and SDS, extracted with a 1:1 mixture of phenol and chloroform and resolved on a 5% native PAGE gel. Junctions were characterized by amplification with primers specific for head to tail junctions (50-CTTACG...