Selected Publications K.G. Zyner, A. Simeone, S.M. Flynn,et al.G-quadruplex DNA structures in human stem cells and differentiation.Nat Commun,2022, 10.1038/s41467-021-27719-1 Read article C. Xu, et al. NPTX2 promotes colorectal cancer growth and liver metastasis by the activation of the...
Immunofluorescence (IF) is also possible with suspension cells after application of the cells to an object slide, e.g. by cytospin. In both cases, the focus is on the analysis of intracellular processes or structures, and this is designated immunocytochemistry (ICC). In immunohistochemistry (IHC...
Pretrained neural network models for biological segmentation can provide good out-of-the-box results for many image types. However, such models do not allow users to adapt the segmentation style to their specific needs and can perform suboptimally for te
Counting populations of live vs dead cells is often performed to assess cell viability. It’s also a good practice to keep track of the percent viability over time, in case something is wrong with your cultures that could also potentially affect your experimental results. A routinely used method...
By the first postnatal week the cells have acquired inward Ca2+ and Na+ currents that enable them to fire spontaneous action potentials at a time when the cochlea can not yet be stimulated by sound. These action potentials may be essential for normal development and survival of the IHCs ...
There are two common classes of fixation; organic solvent methods and the cross-linking method. The goal of both methods is to denature your proteins. Sadly, there is no way to anticipate the best fixation method for your staining or immunohistochemistry needs. Instead, you will likely need to...
Complete sequence of p53 gene in 20 patients with lung cancer: comparison with chemosensitivity and immunohistochemistry. In this study the entire p53 complementary DNA has been sequenced in 20 non-small cell lung carcinomas (NSCLC) and the results correlated with chemosensit... D Brattstr?M,M ...
Sharma M, Yang Z, Miyamoto H, Lucarelli G. Immunohistochemistry of immune checkpoint markers PD-1 and PD-L1 in prostate cancer. Medicine. 2019;98:e17257.https://pubmed.ncbi.nlm.nih.gov/31567999/. Hanahan D, Weinberg RA. The hallmarks of cancer. Cell. 2000;100:57–70.http://www.cell...
PD-L1 immunohistochemistry assays for lung Cancer: results from phase 1 of the blueprint PD-L1 IHC assay comparison project. J Thorac Oncol. 2017;12(2):208–22. 14. Kim H, Choi S, Kim S, Aum J, Pereira S, Park S, et al. Clinical performance of artificial intelligence-powered ...
A total of 109 PD-L1 (Ventana SP142) immunohistochemistry (IHC) stained images were assessed and the role of the AI-assisted model was evaluated. With the assistance of AI, the scoring concordance across pathologists was boosted to excellent in RS3 (0.950, 95% confidence interval (CI): ...