Emily A. MerkelDepartment of Dermatology, Indiana University School of Medicine, Indianapolis, IndianaAhmed K. AlomariDepartment of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IndianaAlly-Khan SomaniDepartment of Dermatology, Indiana University School of Medicine,...
In immunohistochemistry (IHC), on the other hand, the presence of proteins or molecules is examined in a tissue-specific context. Here, ultrathin sections of organ preparations (usually embedded in paraffin wax) are used to e.g. investigate the expression of proteins in a healthy organ compared...
How well do you know your monkeys? 来自 dx.doi.org 喜欢 0 阅读量: 48 作者:D Kaushal,RP Bohm,AA Lackner 摘要: No abstract is available for this article.关键词: baboons immunohistochemistry pathophysiology thoracic endometriosis TTF-1 DOI: 10.1111/jmp.12032 被引量: 2 ...
How to avoid cross-reactivity during immunohistochemistry/immunofluorescence experiments Cross-reactivty might occur in immunostaining assays in tissue samples, for example while working is mouse tissues with mouse-derived antibodies. You can see our blog here on how to tackle mouse-on-mouse staining ...
NOTE:A hemocytometer coverglass is slightly thicker and has a different shape than the type of glass coverslips used for immunohistochemistry and immunocytochemistry assays. If using a reusable hemocytometer, make sure to keep the hemocytometer and the coverglass clean, and handle them carefully because...
Pretrained neural network models for biological segmentation can provide good out-of-the-box results for many image types. However, such models do not allow users to adapt the segmentation style to their specific needs and can perform suboptimally for te
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The chemiluminescent conversion of luminol to 3-aminophthalate is not ideal for immunohistochemistry. The amount of light emitted is relatively low, and it’s all emitted quite quickly, in something akin to a flash. There are several chemicals, mostly phenol derivatives, that rectify this issue.p...
There are two common classes of fixation; organic solvent methods and the cross-linking method. The goal of both methods is to denature your proteins. Sadly, there is no way to anticipate the best fixation method for your staining or immunohistochemistry needs. Instead, you will likely need to...