How are DNA, RNA, and proteins related in the cell? Describe how the DNA code is read through the processes of Transcription and Translation. How do RNA viruses replicate? How does RNA decompose in the cell? What is RNA sequencing and how it is done?
1. Gel electrophoresis is a procedure for separating a mixture of DNAfragments through an agarose gel in an electric field;2. An agarose gel with wells at one end is placed in a buffer containingsalts/ions that will conduct electricity;;3. DNA samples are mixed with loading dye/bromophenol ...
What is the purpose of DNA sequencing? Why is DNA ligase important for a cell? How do you read a gel electrophoresis? What happens when single-stranded DNA goes through gel electrophoresis? What is gel electrophoresis used for? ...
was invented based on the Sanger sequencing principle. In this method the sequencing reaction will be terminated not at the end of the template, but where the protein sits on the DNA, allowing scientists to pin-point the binding site (4). A spin off of that ...
The separated DNA molecules are visualised only agter staining DNA with ethidium bromide followed by exposure to UV radiation, as bright orange coloured bands. The separated bands of DNA (on the gel) are cut from the agarose gel and extracted from the ge
Borst, P., "Ethidium DNA Agarose Gel Electrophoresis: How it started", IUBMB Life, 57(11), 745-747 (2005).P. Borst, Ethidium DNA agarose gel electrophoresis: how it started. IUBMB Life 57 (11), 745−747 (2005)Borst P. Ethidium DNA agarose gel electrophoresis: how it started. IUBMB...
However, a small proportion of the phage DNA is modified prior to degradation by the endonuclease. This modified DNA is able to successfully replicate and infect the second host, but since that host does not contain the same modification system as the first, the modified phage lose their ...
Many Next-Gen Sequencing (NGS) library preparations still require resolving the samples using agarose gels to separate out fragments before sequencing. However, recovering DNA from gel excisions can be challenging with many researchers struggling to recover more than 50% yield from the original input...
Primers for PCR and sequencing should have a GC content between 40 and 60%, with the 3′ of a primer ending in C or G to promote binding. The 3′ end of the primer should be an exact match to the template DNA, because extension by DNA polymerase, during PCR, depends on a good ...
Upon addition of the sugar galactose to yeast cells, nucleosomes in the promoter region of GAL10 are remodeled to activate transcription33. Micrococcal nuclease digestion with deep sequencing (MNase-seq) with high and low MNase concentrations showed the coverage of stable and fragile nucleosomes, re...