Viral evolution experiments were conducted by using a previously characterized HPIV3 neutralizing monoclonal antibody PIA174Ab29to select for variants that are infectious in the presence of the antibody. Two EV viruses emerged (Fig.1A, B). One that we previously characterized functionally (EV-1) be...
Owing to this very strong interaction and the high protein concentrations required in the NMR experiments, we employed fluorescence spectroscopy to avoid ligand depletion effects and to ultimately determine the dissociation constant, Kd, of albicidin binding to AlbAS and AlbAL. The experimental read-...
primarily due to their prolonged generation cycles and high levels of heterozygosity. The advent of CRISPR/Cas editing technology offers a promising solution to these challenges. Successful applications of CRISPR/Cas editing have already been reported in crops such as citrus (Jia and Wang...
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
Flower colour variation is one of the most crucial selection criteria in the breeding of a flowering pot plant, as is also the case for azalea (Rhododendron simsii hybrids). Flavonoid biosynthesis was studied intensively in several species. In azalea, fl
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
Influenza virus can escape most antibodies with single mutations. However, rare antibodies broadly neutralize many viral strains. It is unclear how easily influenza virus might escape such antibodies if there was strong pressure to do so. Here, we map al
The transcriptomes of several cyanobacterial strains have been shown to exhibit diurnal oscillation patterns reflecting the diurnal phototrophic lifestyle of the organisms. The analysis of such genome-wide transcriptional oscillations is often facilitate
[22]. In addition to becoming one of the most commonly used dominant selectable markers in fungal transformation, this work pioneered plasmid mediated cassette integration in filamentous fungal genomes using the vector pAN7-1. Moreover, the authors further validated the use of theA. nidulans...
Endolysins, the cell wall lytic enzymes encoded by bacteriophages to release the phage progeny, are among the top alternatives to fight against multiresistant pathogenic bacteria; one of the current biggest challenges to global health. Their narrow range