To re-engineer them for delivering human genes, scientists "clean out" the harmful parts of the virus, insert a human gene into the virus' genetic material and then inject the virus into the body. Another type of vector is a plasmid, a ring of bacterial DNA into which human genes can ...
Paramyxoviruses including measles, Nipah, and parainfluenza viruses are public health threats with pandemic potential. Human parainfluenza virus type 3 (HPIV3) is a leading cause of illness in pediatric, older, and immunocompromised populations. There ar
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
17.5 may reflect technical rather than true biological variability or represent a mixture of night-activation of gene expression with a global trend, where all transcripts are present at higher levels. Prior to further analysis, the dataset needs to be normalized to distinguish array-to-array noise...
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
After sequence verification, the plasmid was digested with restriction enzymes EcoRI and HindIII to isolate the DNA fragment containing the mutated NT* sequence. The fragment was purified on a 2% agarose gel and ligated into pT7HisTrxHisNT, previously digested with the same enzymes and purified ...
The worldwide emergence of antibiotic resistance poses a serious threat to human health. A molecular understanding of resistance strategies employed by bacteria is obligatory to generate less-susceptible antibiotics. Albicidin is a highly potent antibact
(LigC/D), belonging to theAEP proper cladedefined previously17. The remaining set contains PrimPols with more distant homology. The strongest link between S-2L PrimPol and any other member of the AEP superfamily is with the plasmidic RepB′ (3H20), highlighting the connection between All3500...
[22]. In addition to becoming one of the most commonly used dominant selectable markers in fungal transformation, this work pioneered plasmid mediated cassette integration in filamentous fungal genomes using the vector pAN7-1. Moreover, the authors further validated the use of theA. nidulans...
Flower colour variation is one of the most crucial selection criteria in the breeding of a flowering pot plant, as is also the case for azalea (Rhododendron simsii hybrids). Flavonoid biosynthesis was studied intensively in several species. In azalea, fl