Viral evolution experiments were conducted by using a previously characterized HPIV3 neutralizing monoclonal antibody PIA174Ab29to select for variants that are infectious in the presence of the antibody. Two EV viruses emerged (Fig.1A, B). One that we previously characterized functionally (EV-1) be...
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
primarily due to their prolonged generation cycles and high levels of heterozygosity. The advent of CRISPR/Cas editing technology offers a promising solution to these challenges. Successful applications of CRISPR/Cas editing have already been reported in crops such as citrus (Jia and Wang...
In contrast to what most people might think, citric acid is not—or not anymore—isolated from citrus fruits, but is industrially produced by the filamentous fungusA. niger. The process was pioneered by James Currie, a food chemist, who 100 years ago published a study describing the superior ...
There are restrictions to the availability of the vector and stable cell lines associated with the IGF-1Rzip construct due to the pEE14 vector being subject to a Research Agreement with Lonza. Data and Code Availability MapHO, MapLO, MapHC and MapLC along with their associated atomic models ...
Flower colour variation is one of the most crucial selection criteria in the breeding of a flowering pot plant, as is also the case for azalea (Rhododendron simsii hybrids). Flavonoid biosynthesis was studied intensively in several species. In azalea, fl
Owing to this very strong interaction and the high protein concentrations required in the NMR experiments, we employed fluorescence spectroscopy to avoid ligand depletion effects and to ultimately determine the dissociation constant, Kd, of albicidin binding to AlbAS and AlbAL. The experimental read-...
Influenza virus can escape most antibodies with single mutations. However, rare antibodies broadly neutralize many viral strains. It is unclear how easily influenza virus might escape such antibodies if there was strong pressure to do so. Here, we map al
crystal structure (Protein Data Bank (PDB) ID:4LAV) (ref.46) and adopting different interfaces with GR (Supplementary Movies3and4). In the consensus 3D refinement map, the proline-rich loop adopts a position similar to the crystal structure, and FKBP52A116,S118,P119interact with GRHelix...
The transcriptomes of several cyanobacterial strains have been shown to exhibit diurnal oscillation patterns reflecting the diurnal phototrophic lifestyle of the organisms. The analysis of such genome-wide transcriptional oscillations is often facilitate