as the agar will set in its container before it can be poured. Obtain sterile petri dishes and pour enough to cover the entire surface area of the plate and at least half of its depth. Avoid overfilling the plates and preferably do not allow the agar to contact the top edge ...
Pour 1/4 inch of liquid into each sterile petri dish, making sure to replace the cover immediately. The agar will form a stiff gel at room temperature, and the petri dishes will be ready for use.
Then, eight leaf fragments for each sampled individual (24 fragments per species) were deposited in Petri plates containing PDA (four fragments per plate) supplemented with 250 mg L−1 streptomycin and 100 mg L−1 chloramphenicol, and incubated at 25 °C in the dark for up to five days...
All you need is some agar (a special gelatinous growing material), a number of sterilized Petri dishes, and some disgustingly good sources of bacteria! Part 1 Preparing the Petri Dishes Download Article 1 Prepare the agar. Agar is a jelly-like substance used to culture bacteria. It ...