Liquid nutrient broth is used to culture bacteria such as Escherichia coli. Recipes for this broth vary depending on the bacterial species and the presence of genetic modifications, e.g., antibiotic resistance. The broth can be solidified by adding agar, which enables the bacteria to form distinc...
Place the thermometer in the beaker, and allow the mixture to cool to between 45 and 50 degrees Celsius. Pour 1/4 inch of liquid into each sterile petri dish, making sure to replace the cover immediately. The agar will form a stiff gel at room temperature, and the petri dishes will be...
Purification was carried out by transferring the fungi to Water Agar (WA), followed by incubation at 25 °C for three days. After the incubation period, with the aid of a magnifying glass (Leica M205C®) and sterile needle, small fragments of the culture medium containing an individual ...