The nutrient medium for microorganisms and cell cultures in which volatile and/or toxic substances are contained as carbon source additionally contains suspended active carbon onto which these substances are adsorbed. The nutrient medium can be solidified by addition of agar-agar.ARNTZ HANS-JUERGEN DR...
AKG agar nutrient for the expression of ciliary Salmonella antigens phase, a method for its preparation and the use of the medium to identify the ciliated ... AKG agar nutrient for the expression of ciliary Salmonella antigens phase, a method for its preparation and the use of the medium ...
Preparation of Blood Agar Suspend 28 g of nutrient agar powder in 1 litre of distilled water. Heat this mixture while stirring to fully dissolve all components. Autoclave the dissolved mixture at 121 degrees Celsius for 15 minutes. Once the nutrient agar has been autoclaved, allow it to cool ...
Then each of two copies of 50 μL solutions was taken to inject into itself LB nutrient agar medium plate. An applicator was used to smear it evenly. Then the 5 mm circular sterile filter papers, marked as Nos. 1–5 were placed on the surface of the agar medium plate. The No. 1 ...
The antimicrobial activity was measured by the inhibition zones produced. The inoculum was initially standardized to 108CFU/mL (50% transmittance at 580 nm), by transferring colonies from the nutrient agar to sterile saline, and 200 μL of the suspension was homogeneously placed on the surface of...
The preparation of dietary fiber from wheat straw residual was studied by two extraction methods, NaOH and C2H5OH extraction. Single factor and orthogonal experiments were used to determine the best conditions. Results showed that, for the NaOH extraction of the dietary fiber, the optimum temperature...
Salsola laricifolia is a typical C3–C4 typical desert plant, belonging to the family Amaranthaceae. An efficient single-cell system is crucial to study the gene function of this plant. In this study, we optimized the experimental conditions by using Box
Gellan gum without acyl residue (Gelrite ) is always used as the substitute of agar to solidify nutrient media in thermopiles cultiva- tion19 while gellan gum with low acyl content (Kelcogel F) can be used as a gelling agent in food and personal care applications20. Welan gum, with both ...
Isolation of Protease Producing Microbes from Soy foods Ten grams of natto, a type of common soybean food, were added to 30 ml distilled water, shaken vigorously and held at 75° C. for 20 minutes to destroy vegetative microbial cells. The suspension was then plated on nutrient agar (contain...
microorganisms and fungi; in particular they are active against Staphylococcus aureus, Streptococcus hemolyticus, Diplococcus pneumoniae at doses varying from 0.005 to 0.1 μg/ml. As an example, the following table illustrates the antimicrobial activity of purpuromycin using conventional agar plate ...