In multiple tube operations, in order to prevent mistakes, each student must prepare the following form in the experimental record book. The table is added to each tube volume (ML), and press from left to right, from top to bottom in order by adding tube. The bottom two row is the amo...
To quantify the degree of hydrolysis (DH), which corresponds to the number of cleaved peptide bonds depending on the hydrolysis duration, the ortho-phthaldialdehyde (OPA) method was used [26]. The OPA (Sigma-Aldrich, Oakville, ON, Canada) reagent solution was made from Sodium Dodecyl Sulfate ...