l of 20mM copper nitrate solution in high purity water, and stirring for 2min to 20 min; (2) adding 0.2mL to 3 mL of 1M sodium hydroxide solution into the solution of the step (1); (3) adding 10 mu. l to 100 mu. l of 0.1M reducing agent into the solution of the step (2)...
to yield the compound of formula (I/d): wherein R1, R2, R′3 and R′4 are as defined hereinbefore, or alternatively alkylation on the nitrogen atom in position 5, by means of a reductive amination reaction using a reducing agent such as sodium triacetoxyborohydride or cyanoborohydride in...
530 mg (0.65 mmol) of Example 574 were stirred at room temperature for 18 h in 10 ml of ethanol and 2 ml of 1M sodium hydroxide solution. After addition of water, the alcohol was distilled off. The aqueous solution was acidified with 1.3 ml of 2M HCl. The precipitate was filtered off...
triplicate samples of raw sewage, final effluent and sludge were independently spiked with two different sizes and types of microplastic polymers. Due to the various analytical stages required for the isolation of microplastics, time is a limiting factor in sample processing. The sequential digestion w...
0.9% saline solution from B.Braun (Melsungen, Germany), 3% saline solution from a local pharmacy (Landesapotheke Salzburg, Austria), sodium dihydrogenphosphate-monohydrate and disodiumhydrogenphosphate-dihydrate (both from Merck, Darmstadt, Germany) were used for formulation of the product. [11C]CO2...
4.Lyse the cellsThe cells are lysed with 200-mM NaOH and sodiumdodecyl sulfate(SDS). Thesodium hydroxideraises the pH significantly, resulting in thedenaturationof proteins. TheSDS, beingan ionic detergent, both disrupts cell membranes and binds to thedenatured proteins(seeChapter 3). The protein...
Preparationofbuffersolutions Objective: Tostudythenatureofthebuffers. Introductionandprinciple: Addinga0.01mldropletof1MHClto1LofpurewaterchangesthepHofthewaterfrom 7to5,whichrepresentsa100foldincreasein[H + ].SuchahugechangeinpHwouldbe intolerabletomostbiologicalsystems,sinceevensmallchangesinpHcandramaticallyaf...
formula V is removed and the methyl ester group is converted to a carboxylic acid to give the desired final product of formula VI. For example, when R6is methyl, treatment with methanolic sodium hydroxide followed by treatment with aqueous acid solution gives the desired final product of formula...
salts also was a problem--requiring multiple extractions from a magnesium hydroxide gel. The resultant product had an enantiomeric purity of 99:1 (S:R) by capillary GC (DB-23) of the Mosher amide derivative. ##STR8## The methods reported for preparation of structure 1 (n 1, Ar Ph, R...
0.66 g of dried hemicelluloses were dissolved in 5 mL distilled water at 60 ˝C for 30 min. An aqueous solution of sodium hydroxide (the molar ratio of NaOH to ETA, 0.75) was added, followed by adding of ETA (the molar ratio of ETA to anhydroxylose units in hemicelluloses, 2.0). ...