Skim milk agar plates are used to provide a nutritious medium for growing micro-organisms. Once prepared, the agar can be plated with a population of micro-organisms to test for the micro-organism's ability to digest casein protein. Casein is a large insoluble protein found in skim milk. As...
About LB media Luria-Bertani (LB) is a nutrient-rich liquid broth for growing bacteria. It is commonly used during cloning experiments to amplify competent bacteria. When combined with agar, LB media is also used to createLB agar plateswhich enable the growth of bacteria on a solid medium. ...
as the agar will set in its container before it can be poured. Obtain sterile petri dishes and pour enough to cover the entire surface area of the plate and at least half of its depth. Avoid overfilling the plates and preferably do not allow the agar to contact the top edge ...
Learn how to make a monotub step-by-step and grow mushrooms with this comprehensive monotub cultivation method walkthrough. Find out what supplies you need and the process to follow.
Why do we autoclave/microwave an LB-agar solution, how does it work, and what happens if it is not autoclaved? Compare the recipes of nutrient agar and EMB agar. If an organism can grow on both media, on which would you expect it to better? Expl...
Give 2 reasons why nutrient agar must be cooled to 500 C before inoculating and pouring. How does the amount of each pigment explain why leaves are certain colours? What is the application (purpose) of MacConkey agar? Why are the LB/nutrient agar plates used in selective media and a...
cellobiosus and Lb. plantarum being the initiators of fermentation. During fermentation, the pH drops slowly to a final value of 4 and total acidity (as lactic acid) increase to about 1% (Karki, 1986). Gundruk may be served as a side dish (pickle) or an appetizer. Cucumber is the ...
The ΔmotAB strain moves spores over the entire surface and to the edge of the agar plates in all samples (4.5 cm from the inoculation point) (n = 6) (Figs. 2B and S2). The number of dispersed spores cannot be ascertained due to overlapping colonies that result in apparent ...
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