Skim milk agar plates are used to provide a nutritious medium for growing micro-organisms. Once prepared, the agar can be plated with a population of micro-organisms to test for the micro-organism's ability to digest casein protein. Casein is a large insoluble protein found in skim milk. As...
Luria-Bertani (LB) agar plates are used during cloning using antibiotic-resistant bacteria. Learn how to prepare the plates using this simple recipe.
Measure 500 ml of water into the beaker, followed by the agar powder. The amount of agar powder required per 500 ml can vary depending on the supplier, so check the packaging for instructions on how much powder to add -- 10 to 15 grams is the usual range. This will yield sufficient a...
How to Grow Yeast: How to grow / culture yeast on agar or in YPD: We show you how to prepare fresh cultures of yeast from previously grown agar plates. Spruce up your old yeast cultures by replating onto fresh media or liquid YPD, or select colonies of
4 tsppowdered gelatin or agar powder To use kanten stick, rinse it carefully and break it into pieces to soak in water for 30 minutes. This will help dissolve the kanten faster. Squeeze the water out from the pieces and they are ready to use. ...
You are given a vial of ampicillin that is labeled as a 1% solution and told that you need to make 40 plates using this solution. Assuming that you will need 25 mL , what volume of LB agar solution should you make to prepare the 40 plates? What volu...
Contact plates are convex agar plates that can be directly pressed on to a surface to take a quantitative sample. Contact plates can be made with selective or non-selective agar, with or without a neutralizing agent, all of which lead to differences in recovery of the target organism. The ...
Agar plates used for manual ASTs are interpreted by some manufacturers as Class A tests [17]. The Clinical & Laboratory Standards Institute (CLSI) M52 and ISO 20776-2:2021 on verification of ASTs, focus on automated AST [18] and MIC-producing ASTs [19], respectively. The latter ...
the 60 Petri dishes (See Appendix 2). In order to ensure that the entire surface area of thedishwas covered‚ the E. Coli bacteria culture was swabbed in at least 3 different directions. The different types of antibiotics were applied to each of the agar plates by using five sterile for...
GEM cDNA clones (supplied as a bacterial stab) were purchased from IncyteGenomics and streaked on LB agar plates containing the appropriate antibiotic. Individual colonies were picked and grown in LB medium. Plasmid DNA was isolated and sequenced in order to verify the sequence identity. Northern ...