Basic components: Tris-HCl (buffer component to prevent protein denaturation), NaCl (salt component to prevent non-specific protein aggregation), NP-40 (non-ionic detergent for protein extraction, prepared as a 10% stock solution in water), sodium deoxycholate (ionic detergent for protein extraction...
How to prepare 1 ml of GTE buffer (50mM glucose,25mM Tris Hcl PH 8.0,10mM EDTA) for the plasmid isolation from the given stock solutions (2Mm Tris-Hcl ,0.5mM EDTA,1M glucose)? You have a 5.5 M solution of Tris buffer pH 7.9. ...
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Solution I: 25 mM TrisHCl, pH 8.0; 50 mM Glucose Solution II: 0.2 N NaOH; 1% SDS Solution III: 5 M Potassium Acetate, pH 4.8. Add glacial aceticacid to a solution of 3 M potassium acetate to achieve a pH 4.8.PREPARATION OF SOURCE DNA LIGATION AND ELECTROPORATION BAC LIGATION DNA sho...
Which buffer system is the best choice to create a buffer with pH=7.20? For the best system, calculate the ratio of the masses of the buffer components required to make the buffer. HC2H3O2/KC2H3O2 H If you were conducting an experiment with pepsin, which has optimal en...
A few attempts have been made to make such physical models [6] such as "Positional-Dependent-Nearest-Neighbour model" (PDNN, [7]), or a combination of the two model types GeneChip and RMA (GC-RMA, [8]). A specific feature of Affymetrix microarrays are the so-called MisMatch (MM) ...
All enzyme storage conditions are validated through our Quality Assurance re-assay program to maximize long term stability. Setting up digests with a single restriction enzyme is relatively straightforward. However, digests using multiple enzymes that have different buffer requirements may demand the use...
Reactions were incubated at 37°C for 15 minutes, and the residual amidolytic activity was determined by the addition of 0.2 mM S-2765 substrate in assay buffer (50 mM Tris-HCl, 100 mM NaCl, pH 7.4, with 0.1% polyethylene glycol 8000, 0.1 mg/mL bovine serum albumin). Linear regression...
Explain how to prepare 250 ml of 0.2 M phosphate buffer of pH 6.4.starting from Na2HPO4, NaH2PO4, 0.129 N NaOH solution. Acid-base Buffer Solutions: Dissolution of a weak acid and the conjugate base of that weak acid into the same solu...
Calculate how many grams you need to make a 20 mM buffer of Tris/ 1mM EDTA buffer? (1360 mL final volume) Tris:121.14 g/mol. EDTA:292.24 g/mol Calculate the amount of , required to prepare 500 mL of 0.01 M EDTA solution. How wou...