How to prepare 1 ml of GTE buffer (50mM glucose,25mM Tris Hcl PH 8.0,10mM EDTA) for the plasmid isolation from the given stock solutions (2Mm Tris-Hcl ,0.5mM EDTA,1M glucose)? You have a 5.5 M solution of Tris buffer pH 7.9. ...
Which buffer system is the best choice to create a buffer with pH=7.20? For the best system, calculate the ratio of the masses of the buffer components required to make the buffer. HC2H3O2/KC2H3O2 H If you were conducting an experiment with pepsin, which has optimal e...
Basic components: Tris-HCl (buffer component to prevent protein denaturation), NaCl (salt component to prevent non-specific protein aggregation), NP-40 (non-ionic detergent for protein extraction, prepared as a 10% stock solution in water), sodium deoxycholate (ionic detergent for protein extraction...
aHalf livers were rinsed in ice-cold physiological saline and homogenized in Tris–HCl buffer (0.01 M, pH = 7.4) to give a 10% homogenates. Homogenates were centrifuged at 3000 rpm, 4 °C for 10 min and supernatants were collected for catalase (CAT), total superoxide dismutase (T-SOD) ...
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with good aeration overnight. Make sure to take a blue colony on an X-gal/IPTG plate. 2)Harvest the cells by centrifugation, and resuspend the cell pellet in Solution I (without lysozyme). Use 25 ml Solution I per liter culture.
All enzyme storage conditions are validated through our Quality Assurance re-assay program to maximize long term stability. Setting up digests with a single restriction enzyme is relatively straightforward. However, digests using multiple enzymes that have different buffer requirements may demand the use...
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Explain how to prepare 250 ml of 0.2 M phosphate buffer of pH 6.4.starting from Na2HPO4, NaH2PO4, 0.129 N NaOH solution. Acid-base Buffer Solutions: Dissolution of a weak acid and the conjugate base of that weak acid into the same solu...
Calculate how many grams you need to make a 20 mM buffer of Tris/ 1mM EDTA buffer? (1360 mL final volume) Tris:121.14 g/mol. EDTA:292.24 g/mol Calculate the amount of , required to prepare 500 mL of 0.01 M EDTA solution. How wou...