The knock-out technique remains a widely utilized approach in gene editing, particularly for introducing modifications in gene coding regions. This method involves inducing frameshift mutations, altering intron splice junctions to encourage intron skipping, or creating large fragment deletions (Zhang et al...
With the intensification of the greenhouse effect, a series of natural phenomena, such as global warming, are gradually recognized; when the ambient temperature increases to the extent that it causes heat stress in plants, agricultural production will in
NAC family TFs proteins target different categories of genes involved in various stress responsive processes through membrane-nuclear translocation, completing responses to abiotic stress factors. Heat stress induces ONAC023 nuclear translocation for thermosensing. The dephosphorylation of the remorin protein ...
cerevisiae , to the formation of double-strand DNA breaks during meiosis. Article CAS PubMed Google Scholar Luijsterburg, M. S., White, M. F., van Driel, R. & Dame, R. T. The major architects of chromatin: architectural proteins in bacteria, archaea and eukaryotes. Crit. Rev. ...
Interestingly, the combined knockout of Nr1i2 and the gene encoding the LPS sensor toll-like receptor 4 (Tlr4) can rescue the phenotype of Nr1i2−/− mice, possibly because it counteracts the accelerated cell death of inflammatory monocytes observed in NR1I2-deficient mice (Qiu et al....
The study of skeletal muscle-specific glucocorticoid receptor knockout mice revealed that the depletion of plasma alanine due to increased muscle mass leads to a rise in plasma levels of FGF21, activating liver-fat communication and influencing lipolytic genes in adipose tissues [44]. The ...
human studies that support the idea that income inequality is an underlying factor for the maladaptive changes seen in the microbiota in certain populations. We propose that this contributes to the health disparities that are seen between lower-income and higher-income populations in high-income ...
as molecular scissors, where a simpleexchange of the RNA guide sequence is enough to redesign the nucleasespecificity has paved the way for a revolution in the life sciences. CRISPR repeats are associated with Casproteins constituting an adaptive immune system in bacteria and archaeaprotecting them ...
The authors found that the high expression of DC-related genes in the tumor tissues of patients with kidney cancer and NSCLC before treatment is positively correlated with OS [72]. TIM3 is also expressed in the DC. In one study, after tim-3 knockout in DCs, the number of infiltrating ...
On the contrary, even after αGal was found to be the main cause of HAR, the knockout of αGal was not easy. This is because unlike in mice, porcine embryonic stem cells have not yet been established. Therefore, competitive inhibition and remodeling of Gal epitopes by the overexpression of...