Cas13d has also been used to efficiently knock out genes involved in gene amplification, apoptosis, metabolism, and glycosylation in Chinese hamster ovary (CHO) cells, which are used for recombinant protein production in the biopharmaceutical industry. It has also been utilized in microbial RNA knoc...
The knock-out technique remains a widely utilized approach in gene editing, particularly for introducing modifications in gene coding regions. This method involves inducing frameshift mutations, altering intron splice junctions to encourage intron skipping, or creating large fragment deletions (Zhang et al...
NAC family TFs proteins target different categories of genes involved in various stress responsive processes through membrane-nuclear translocation, completing responses to abiotic stress factors. Heat stress induces ONAC023 nuclear translocation for thermosensing. The dephosphorylation of the remorin protein ...
cerevisiae , to the formation of double-strand DNA breaks during meiosis. Article CAS PubMed Google Scholar Luijsterburg, M. S., White, M. F., van Driel, R. & Dame, R. T. The major architects of chromatin: architectural proteins in bacteria, archaea and eukaryotes. Crit. Rev. ...
With the intensification of the greenhouse effect, a series of natural phenomena, such as global warming, are gradually recognized; when the ambient temperature increases to the extent that it causes heat stress in plants, agricultural production will in
Next article in issue Keywords Sex difference Gut microbiota Microbiome Sex hormone Human lifespan Diet Antibiotic Lifestyle Environment 1. Introduction The “gut microbiota” is a term referring to the assembly of bacteria, archaea, viruses, and fungi that reside in the gastrointestinal (GI) tract...
human studies that support the idea that income inequality is an underlying factor for the maladaptive changes seen in the microbiota in certain populations. We propose that this contributes to the health disparities that are seen between lower-income and higher-income populations in high-income ...
Researchers at the University of Toronto have discovered that an overlooked gene plays a major role in the development of antibodies, which help the immune system recognize and fight viruses including SARS-CoV-2, bacteria and other causes of infectious d
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On the contrary, even afterαGalwas found to be the main cause of HAR, the knockout of αGal was not easy. This is because unlike in mice, porcine embryonic stem cells have not yet been established. Therefore, competitive inhibition and remodeling of Gal epitopes by the overexpression of ...