基因敲除Gene knockout Geneknockout Lecturer:DuShengyangJanuary242013 Minimumgenome BasedonakindofidealhypothesisItassumethatcellscontainaminimalgenesetUndertheconditionofthegenesettomaintainnormallifefunctions reduced genome Graduallyeliminatenonessentialgenesequence Thesignificanceofgeneknockout Gene...
Scarlett DJ, Herst P, Tan A, Prata C, Berridge M: Mitochondrial gene-knockout (rho0) cells: a versatile model for exploring the secrets of trans-plasma membrane electron transport. Biofactors. 2004, 20 (4): 199-206. Article PubMed Google Scholar Chandel NS, Schumacker PT: Cells deplet...
A lentiviral vector encoding the human Wiskott–Aldrich syndrome protein corrects immune and cytoskeletal defects in WASP knockout mice. Gene Ther 2004; 12: 597–606. 49 Themis M, Waddington SN, Schmidt M, von Kalle C, Wang Y, Al-Allaf F et al. Oncogenesis following delivery of a non...
Past studies, using CCR2 knockout mice, have clearly demonstrated that CCR2 via binding to its ligand MCP-1 present in the inflamed sites is essential for recruitment of Gr+ positive monocytes to the inflamed tissues.47 The high level of CCR2 expression in both CD11b+Gr1+ monocytes and ...
In accordance with this finding, they also did not observe TPA-induced S100A9 gene expression in parp1 knockout mice. Therefore, we investigated S100A9 gene expression in HaCaT keratinocytes in the absence and presence of the PARP-1 inhibitor 1,5-isoquinolinediol (DiQ). Both TPA- and TNFα...
In order to trace the effects that COSMOC knockout could have on the transition from pluripotency to neural/neuronal stages, we next used the CRISPR/Cas9 technology and successfully derived two different knockout (COSMOC−/−) hiPSC lines (Supplementary Fig. 5d, e). COSMOC−/− hiPSCs...
(Ang-RBCm) as out-layer. The unique three-layer core–shell structure nanocomplex demonstrated increased blood–brain barrier (BBB) transcytosis, extended blood circulation time, and efficient tumor-selective targeting. Particularly, after the endocytosis of Ang-RBCm-CA/siRNA, the PLL-CA layer can...
been refuted by the analysis of large FRDA patient cohort data and by revisiting the liver tumor pathology in the conditional knockout frataxin mouse model.31Frataxin has been proposed to be a tumor suppressor gene by Gucciniet al.,32who showed that frataxin modulates P53 expression in tumors ...
The same methodology used to knock out CMAH in mice is easily adapted for disruption of CMAH gene expression in domesticated animals, because of the high level of homology between CMAH genes in all mammals. For mice, a frameshift mutation, similar to the one found in the human CMAH, was...
For example, the knockout of the GPR45 genes or the reduction of the expression of the GPR45 genes can be realized through insertion of an exogenous DNG segment such as a transposon or a virus in a GPR45 gene sequence or through gene manipulation mediated by a method, e.g., a chemical...