et al. (1993) Use of specific oligonucleotide duplexes to stimulate cleavage of refractory DNA sites by restriction endonucleases. Anal. Biochem. 209, 232–7. Oller, A.R. et al. (1991) Ability of DNA and spermidine to affect the activity of restriction endonucleases from several bacterial ...
B. et al. Surface expression of Toll-like receptor 9 is upregulated on intestinal epithelial cells in response to pathogenic bacterial DNA. Infect. Immun. 75, 2572–2579 (2007). Article CAS PubMed PubMed Central Google Scholar Moran, G. W., Leslie, F. C., Levison, S. E. & ...
Inquiry should be forwarded to: Ung-Jin Kim or Hiroaki Shizuya.PREPARATION OF BAC VECTOR DNA Because BAC vectors are single copy plasmids, it is rather difficult to obtain large amount of BAC vector DNA. Extra care is also needed to minimize the contamination of E. coli DNA that consists mo...
DNA, BacterialReagent Kits, DiagnosticPlasmidsTo isolate plasmid DNA from bacteria using a commercial plasmid miniprep kit (if interested, compare this protocol with Isolation of plasmid DNA from bacteria).doi:10.1016/B978-0-12-418687-3.00002-1Laura Koontz...
Such models are often trained on existing task-specific datasets and subsequently used to generalize to unseen data. The ability to harness large datasets for AI-guided decision-making can revolutionize clinical diagnosis of bacterial infections, AMR surveillance, and antibiotic discovery4,5. For ...
3Genetic basis ofS. suisresistance to antibiotics The mode of action of the above-mentioned antibiotics is class-dependent, as schematized in Figure2. Overall, antibiotics obstruct vital biological processes, such as cell-wall synthesis, protein synthesis, or DNA replication, mainly by interfering ...
Explain how a nucleic acid probe can be used to identify a specific gene. (Think of bacterial transfer). Gene: DNA is the material that makes up genes. A gene refers to the functional unit of DNA that contributes to phenotype or function. They hold encod...
could even enter bacterial cells to damage their DNA. They wondered if a third party — bacteria-infecting viruses — might be involved. Hardly more than bits of genetic information, these viruses can slip into bacteria’s DNA and lie quietly in wait. Then, once triggered, they cau...
How does DNA replication work? Does prokaryotes or archaea have a mitochondria? In microbiology, how does binary fission happen? How is bacterial DNA replication different from eukaryotic DNA replication? How does the nucleus control protein synthesis in the cytoplasm?
Over the past few years, culture-based methods and quantitative real-time polymerase chain reaction (qRT-PCR) on DNA extracted from colorectal tissue biopsies, as well as patients’ stool, have allowed us to identify enrichments in particular bacterial species. In addition, present-day next-genera...