Protein-protein interactions (PPIs) play vital roles in life and provide new opportunities for therapeutic interventions. In this large data analysis, 3,300 inhibitors of PPIs (iPPIs) were compared to 17 reference datasets of collectively ~566,000 compounds (including natural compounds, existing drug...
The brainchild of Stanley Fields, a Howard Hughes Medical Institute investigator at the University of Washington, Y_2H is a genetic method that relies on the modular architecture of transcriptional activators to identify protein-protein interactions in the yeast...
Chromosome conformation capture (3C) methods are central to understanding the link between nuclear structure and function, and the physical interactions between distal regulatory elements and promoters. However, no one method is appropriate to address all biological questions, as each variant differs marke...
Most of the cellular functions are the result of the concerted action of protein complexes forming pathways and networks. For this reason, efforts were devoted to the study of protein-protein interactions. Large-scale experiments on whole genomes allowed
Introduction: The N-terminus of a protein can encode several protein features, including its half-live and its localization. As the proteomics field remains dominated by bottom-up approaches and as N-terminal peptides only account for a fraction of all analyzable peptides, there is a need for...
Mayya V, Lundgren DH, Hwang SI, Rezaul K, Wu L, Eng JK, Rodionov V, Han DK (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modu- lation of protein-protein interactions. Sci Signal 2(84):rs46. https ://doi.org/10.1126/scisignal....
In this study, Noma and his colleagues identified the interactions of proteins that link condensin and mitosis. By studying mitosis in yeast, they were able to identify a subunit of condensin called Cnd2 that binds directly to a protein called the TATA box-binding protein (TBP). TBP is a...
Zhang L, Miles MF, Aldape KD: A model of molecular interactions on short oligonucleotide microarrays. Nature Biotechnology 2003, 21: 818–821. 10.1038/nbt836 Article CAS PubMed Google Scholar Wu Z, Irizarry RA: Preprocessing of oligonucleotide array data. Nature Biotechnology 2004, 22: 656–658...
Microbes catabolize proteins through the action of protease enzymes they produce. This ability is used to identify bacteria based on the specific type... Learn more about this topic: Protein Synthesis | Definition, Diagram & Process from
et al. RNA binding protein/RNA element interactions and the control of translation. Curr. Protein Pept. Sci. 13, 294–304 (2012). Article CAS PubMed PubMed Central Google Scholar Manning, K. S. & Cooper, T. A. The roles of RNA processing in translating genotype to phenotype. Nat. ...