Explain how gel electrophoresis is used to analyze nucleic acids and to distinguish between two alleles of a gene. Explain how genetic information is transferred from DNA to RNA to proteins. Include the terms DNA replication, transcription, translation, the principal events and...
Explain how the ability to isolate DNA and run gel electrophoresis of DNA relates to biotechnology. DNA DNA is the heritable molecule of life. This molecule is a double-stranded helix comprised of two strands of nucleotides. The tw...
Explain how gel electrophoresis is used to sort DNA and proteins. What is the role of DNA polymerase III? How do people mass produce an enzyme like polymerase and reverse transcriptase? How did the discovery of the enzyme DNA polymerase play in the understanding for the process of DNA replicat...
One important result is that carbon rather than nitrogen or phosphorus starvation leads to the development of a starvation and stress resistant cell in these organisms. Hence, we have attempted to characterize the carbon starvation stimulon. By the use of two-dimensional gel electrophoresis of pulse...
Thus, none of the physico-chemical parameters here considered seems to be able to explain or predict the CeO2 stamps’ genotoxic effect. It is important to highlight that CeO2 stamps were the only nanoform produced in the presence of HMT, which might have toxic properties. Indeed, HMT-CeO2...
Phage isolated from these plaques were able to re-infect the second strain and grow well, but lost the ability to grow on the original strain. Arber and Dussoix (5,6) proposed a molecular model to explain host-controlled restriction modification. They postulated that certain bacterial strains...
yield and purity of the amplified DNA sequencesusing gel electrophoresis, spectrophotometry or fluorometry. However, you can’t determine the amount of template DNA present in a sample before amplification using standard PCR. If this is a requirement for your experiment, you have to perform a qPCR...
One often has to work from the assumption or prior knowledge that specific proteins are phosphorylated and investigate this using approaches such as cloning, 1D or 2D gel electrophoresis combined with Pro-Q Diamond staining [182–190], radioactive labelling using 32P or 33P [182, 183], or ...
How do you signify adding water to perform the recrystallization procedure in a balanced equation? Explain why you prepare 3 dilutions of a sample. Explain the difference between a solution, a solvent, and a solute. Explain how gel electrophoresis works. ...
Explain how gel electrophoresis works. How do we use nitrogen in our biological processes? What are the three major steps in PCR and what happens in each step? What's PCR, How does it work, and what can it be used for? How do surfactants work?