However, Schwartz and Cantor (1984) showed that it was possible to resolve much larger fragments by varying the orientation of the electric field in the gel during the run. Each time the direction of the electric field is switched, the molecule is forced to change to a new conformation that...
Electrophoresis is often classified according to the presence or absence of a solid supporting medium or matrix through which the charged molecules move in the electrophoretic system. The fundamental driving force of electrophoresis is the voltage applied to the system. Gel electrophoresis is a ...
7.3.5 实验室视频:凝胶电泳(Lab Video: Gel Electrophoresis) 05:29 8.1.1 基因组学-人类基因组介绍(Genomics - Human Genome Introduction) 01:04 8.1.2 重组DNA回顾(Recombinant DNA Review) 08:01 8.1.3 寻找你的基因:人类孟德尔疾病(Finding Your Gene: Human Mendelian Diseases) 11:25 8.1.4 人类基因...
7.3.5 实验室视频:凝胶电泳(Lab Video: Gel Electrophoresis) 05:29 8.1.1 基因组学-人类基因组介绍(Genomics - Human Genome Introduction) 01:04 8.1.2 重组DNA回顾(Recombinant DNA Review) 08:01 8.1.3 寻找你的基因:人类孟德尔疾病(Finding Your Gene: Human Mendelian Diseases) 11:25 8.1.4 人类基因...
Some of these analyses rely on blotting techniques, which introduce another variable due to nucleic acid transfer efficiencies, while other assays were developed to eliminate the need for gel electrophoresis yet provide the requisite specificity. Real-time PCR, which provides the ability to view the ...
Teske, A., P. Sigalevich, Y. Cohen, and G. Muyzer. 1996 Molecular identification of bacteria from a coculture by denaturing gradient gel electrophoresis of 16S ribosomal DNA fragments as a tool for isolation in pure cultures Appl. Environ. Microbiol. 62 4210–4215 ...
(sodium dodecyl sulfate-polyacrylamide gel electrophoresis), a pH-optimum of pH 5.5, a pl of 3.9, and its activity was found to be insensitive to EDTA; (b) the enzyme splits single-stranded RNA endonucleolytically by a phosphotransferase reaction yielding 2',3'-cNMPs as primary monomeric ...
complementary strand of the artificial adapter as a primer. Subsequently, 1-3 selective bases are added to the pre-amplified products as primers for selective amplification. Polyacrylamide gel electrophoresis is used for separation, and polymorphisms are detected based on differences in amplified ...
In order to precisely localize enzymatic activity in a tissue, cell, gel or blot, the substrate must yield a product that immediately precipitates or reacts at the site of enzymatic activity. In addition, various methods such as chromatography, isoelectric foc...
The frequencies of nucleotide insertion opposite each of the three lesions and the frequencies of chain extension from the 3'-primer terminus, determined by kinetic analysis, were consistent with results of experiments utilizing polyacrylamide gel electrophoresis. We conclude from these studies that ...