To confirm that the biological sample matrix is not masking or enhancing the signal, spike-and-recovery and linearity-of dilution experiments should be performed. 5) The Detection Antibody Concentration Prepare different concentrations of the detection antibody. 6) The Enzyme Conjugate Concentration ...
Those using the phospho-CD247 antibody would likely be studying T cell activation and CAR T cells. The zeta chain of the CD3 complex is the part responsible for initiating the signaling cascade upon engagement of the T cell receptor. It is phosphorylated on Tyr142 upon TC...
Therefore, secreted cytokines are captured by the immobilized antibody on the PVDF surface. After washing, a biotinylated detection antibody is added to allow detection of the captured cytokine and the secreted cytokine is visualized using an avidin-HRP or avidin-ALP conjugate and a colored ...