Most commonly, cryo-concentration, where your protein is not evenly distributed throughout your frozen container, poses a risk to critical product quality attributes. There are countless ways improper freeze/thaw processes can ruin sensitive therapeutics and cell and gene therapies, but the proper ...
The position of unfolding curves of oligomeric proteins depends on the protein concentration. The extent of this dependence is analyzed here in terms of the midpoint concentration, i.e., the denaturant concentration at which the fractions of folded and unfolded protein are equal. Reexamination of...
Germ cells are the only cells in the body capable of giving rise to a new organism, and this totipotency hinges on their ability to assemble membraneless germ granules. These specialized RNA and protein complexes are hallmarks of germ cells throughout their life cycle: as embryonic germ granules...
When looking at an ELISA kit’s specifications, remember to check for any cross-reactivity information provided by the manufacturer. Kit Sensitivity Do you have a general idea about the concentration of the target analyte in your sample? If you have no clue about the concentration, it might be...
Absence of these amino-acids will result to weakness, poor concentration and memory, poor muscle building, mood swings, unbalanced blood sugar levels, and trouble maintaining or losing weight. A case study by PubMed.govexplained deficiency of protein S as a life-threatening problem in the body’...
CheKine™ Blood Calcium Colorimetric Assay Kitprovides a simple test method to detect the free calcium content in plasma. This kit is used to detect the concentration of free calcium in the blood. In the determination, the free calcium in the plasma reacts with GBHA in a strong alkaline so...
1) Capture Antibody Concentration Prepare different concentrations of the capture antibody in coating buffer. 2) The Blocking Buffer Prepare different blocking solutions. If the blocking solution is not preformulated (i.e., it is a single protein, such as BSA), try different concentrations of th...
Using this method, the density of ligand immobilized to an SPR sensor can be increased substantially using only a small initial concentration of ligand. This means that an SPR experiment can be performed using even less of your protein sample. For even smaller sample sizes, check out Alto™ ...
PBS++ contains 1 mM CaCl2 and MgCl2, which is presumed to have a membrane-stabilizing effect preventing cell detachment. For PBS-T a final concentration of 0.05 % of the detergent Tween 20 is added with the aim of increasing the binding specificity of the antibodies. It is extremely ...
the first is that the medium at which drugs work is mainly aqueous. Low solubility in aqueous media will drastically lower the concentration of the drug causing a poor bioavailability. Nanoparticles can provide an alternative medium for these drugs to be solubilized in and carried out through the...