how to design and optimize pcr primers and sequence-specific oligonucleotide probes for dna typingdoi:10.1016/0197-1859(93)90003-3Hurley, Carolyn K.Kukuruga, DebraRosen Bronson, SandraClinical Immunology Newsletter
sequencing by CE is used to determine the specific base sequence of a particular fragment or gene segment, this technique can also provide sizing, relative quantitation, and genotyping information for fluorescently labeled DNA fragments produced by PCR using primers desi...
Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain reaction (qPCR), you should always run a standard curve to calculate the efficiency of your PCR primers. ...
Human DNA polymerase θ grasps the primer terminus to mediate DNA repair. Nat. Struct. Mol. Biol. 22, 304–311 (2015). Article CAS PubMed PubMed Central Google Scholar Mateos-Gomez, P. A. et al. Mammalian polymerase θ promotes alternative NHEJ and suppresses recombination. Nature 518, ...
Looking for resources to help you prep for the Coding Interview?Check out the sister repo Interactive Coding Challenges, which contains an additional Anki deck:Coding deck ContributingLearn from the community.Feel free to submit pull requests to help:...
wang247 / system-design-primer Public forked from donnemartin/system-design-primer Notifications You must be signed in to change notification settings Fork 0 Star 0 Learn how to design large-scale systems. Prep for the system design interview. Includes Anki flashcards. License...
RNA molecules can fold into intricate shapes that can provide an additional layer of control of gene expression beyond that of their sequence. In this Review, we discuss the current mechanistic understanding of structures in 5′ untranslated regions (UTRs) of eukaryotic mRNAs and the emerging metho...
The Fibonacci sequence is a set of steadily increasing numbers where each number is equal to the sum of the preceding two numbers. The golden ratio of 1.618 is derived from the Fibonacci sequence. Many things in nature have dimensional properties that adhere to the golden ratio of 1.618. ...
Healthcare and scientific research: Scientists can use AI to model protein sequences, discover new molecules, or suggest new drug compounds to test, while doctors and practitioners can use AI to analyze images to aid in diagnoses. Media and entertainment: AI can be used to quickly, easily, an...
Why bother with PCR primer efficiencies? Every time you receive a new set of primers, especially when using SYBR Green chemistry during quantitative polymerase chain reaction (qPCR), you should always run a standard curve to calculate the efficiency of your PCR primers. ...