High-throughput sequencing assays have become an increasingly common part of biological research across multiple fields. Even as the resulting sequences pile up in public databases, it is not always obvious how to make use of these data sets. Functional genomics offers approaches to integrate these...
This guide introduces restriction enzymes, providing in-depth reference information and tools to help you find buffers for double digests, or find enzymes by name or recognition sequence. Restriction Enzyme Tools are available for desktop or mobile....
Primers for PCR and sequencing should have a GC content between 40 and 60%, with the 3′ of a primer ending in C or G to promote binding. The 3′ end of the primer should be an exact match to the template DNA, because extension by DNA polymerase, during PCR, depends on a good ...
primers ensuring that if a probe is present, it will more likely be the matched probe. This implies that, while promoting specificity, genotyping assays cannot be used for quantification. Genotyping assays aim to discriminate between sequences, rather than mea...
How do you determine how to make a sequencing primer? What is alkalosis and how does it develop? What are the primary effects? Explain the Niche Overlap Hypothesis. How do I create a concept map for anemia of chronic diseases? Why is taxonomy important in biology?
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The information role of DNA was further supported in 1952 when Alfred Hershey and Martha Chase demonstrated that to make new viruses, a bacteriophage virus injected DNA, not protein, into the host cell. So, scientists had theorized about the informational role of DNA for a long time, but no...
This learning is facilitated by convolutional layers that apply filters to local patches of input data, pooling layers that reduce dimensionality, and fully connected layers that interpret the features extracted to make decisions. In the field of biology, CNNs have proven invaluable for tasks ...
"Genome sequencing and other projects started generating massive amounts of training data, and advances in computing made it possible to store and access this data. We could train the machines to do more complex tasks. You couldn't have had a deep learning model 30 years ago, because you ...
Was he the first one to make the discovery, or did he just spend the most time on it? What happens if the wrong primers are used in PCR? Will the reaction proceed or halt at a certain point, and what stage of the process does it impair? What are the potential consequences of this...