qPCR requires a fluorescent intercalating dye or fluorescently-labeled probes, and a thermal cycler that can measure fluorescence and calculate the cycle threshold (Ct) value. Typically, the fluorescence intensity increases proportionately to the concentration of the PCR product being formed, measuring qua...
Free PCR and qPCR design tools! IDT offers several free online tools (SciTools Web Tools) for oligonucleotide design and analysis. These tools contain design engines that use sophisticated formulas that, for example, take into account nearest neighbor analysis to calculate Tm, and allow you to be...
The PM and MM probe of one probe pair are located physically next to each other on the microarray. Both MAS5 and dChip in the PMMM mode use this information to calculate gene expression. However, in the literature there is a debate over the usefulness of MM probes, which has resulted ...
It is possible to calculate the expected average fragment size for a given genomic DNA if the percent GC content of the DNA and the recognition sequence of the restriction enzyme are known. For example, in a genome with 50% GC content and no dinucleotide bias, a four-cutter can be expecte...
Bioinformatics - From Genomes to Therapies: Volumes 1-3 part 1 绝对珍贵的资源~~~我找了好久才找到~~在此分享~~ 这是Wiley上的电子书 一般是下不到的~~~ Bioinformatics - From Genomes to Therapies: Volume 1: The Building Blocks: Molecular Sequences and Structures; Volume 2: Getting at the Inne...
Disease severity values were used to calculate the AUDCP values (area under the disease progress curve) according to the following formula [44]: AUDPC = Σ((yi + yi+1)/2) × (ti+1 − ti) where yi is the value of the disease severity for the “i” observation, and ti is the...
It is possible to calculate the expected average fragment size for a given genomic DNA if the percent GC content of the DNA and the recognition sequence of the restriction enzyme are known. For example, in a genome with 50% GC content and no dinucleotide bias, a four-cutter can be expecte...