How Trp repressor binds to its operator [published erratum appears in EMBO J 1990 Sep;9(9):3023]Staacke, DWalter, BKistersWoike, BVon WilckenBergmann, BMullerHill, B
The concentration of newly synthetized dPER and dTIM proteins stays low to begin with, since protein accumulation is delayed by their proteasomal degradation. dPER degradation is initiated by phosphorylation through DOUBLETIME (DBT), a homolog of casein kinase I (CKI), which leads to the ...
How is negative regulation lifted in the lac operon? a. Lactose levels fall in the cell. b. Glucose levels fall in the cell. c. The Lac Repressor protein undergoes a conformational change when lactose is present. d. CRP recruits RNA polymerase to the pr ...
Regulationofcold-responsivetranscriptomebyCBFs4.ICE1,amasterregulatorofcoldacclimation5.Self-regulationofCBFexpression6.Repressorsofcold-inducedtranscriptome7.CBF-independentpathwaysofcoldresponses 1.ColdperceptionmechanismsandpotentialLTsensors PlasmamembraneisimportantforperceptionorsensingtheLTstress.
2. Post-Translational Modifications in Selected Cellular Events of the Diverse Pathomechanism of Huntington’s Disease 2.1. PTMs in Abnormal HTT Protein Aggregation HTT is a large, 348 kDa protein. The disease-causing polyQ stretch is found within exon-1 encoding the N-terminal fragment of the...
Aggregation of the protein fused with fluorescent protein is monitored with microscopy. The fusion of protein of interest with functional domain of Sup35 allows verify aggregation on the phenotypic level. Inactivation of the POI-Sup35MC into aggregates leads to the nonsense-suppressor phenotype which...