After adding 10 mL of IPTG the incubation was continued (250 rpm, 37 °C) for 3 h. At the end of the induction the suspension has been trans- ferred in two 1.5 L triangular bottles and spun down in the Centrifuge CR30NX with Rotor R9A2 (4,000 rpm, 30 min, 4°C). The ...
coli BL21 cells, and for the purification of the enzyme we used an IPTG inducing expression plasmid with a glutathione tag. For the purification we applied the protein onto a glutathione-sepharose matrix. An overnight culture of the cells (250 mL LB medium with 2% glucose, ampicillin 100 ...