Y263F and R305K mutants in NarK completely abolished transport activity even under high isopropyl-β-D-thiogalactoside (IPTG) concentration, which is used to induce protein expression (Fukuda et al., 2015), whereas the R89K mutant rescued the NO3−-uptake activity under the highest IPTG ...
In this work, we identified the disruptive effect of fluctuating air/liquid interfaces, potentiated by solid/liquid interfaces and shear stress under agitation in complex protein solutions. This effect is stronger for large protein complexes, yet small globular proteins are also affected. It is suffic...
The 5G4 antibody, which does not recognize α-syn monomers, also showed strong immunoreactivity toward HNE-induced oligomers but exhibited weak immunoreactivity toward DA-induced oligomers, which could be due to the presence of a small population of structured DA oligomers (Table 4) or due to ...
coli BL21 cells, and for the purification of the enzyme we used an IPTG inducing expression plasmid with a glutathione tag. For the purification we applied the protein onto a glutathione-sepharose matrix. An overnight culture of the cells (250 mL LB medium with 2% glucose, ampicillin 100 ...