Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
This study has analyzed the presence of a furin-like cleavage site in the S protein of recently emerged coronaviruses, SARS-CoV-2, SARS-CoV, and MERS-CoV, using the Clustal omega tool. The results confirmed its absence in SARS-CoV and its presence in the other two coronaviruses (Fig. ...
Method choices include Clustal Omega, ClustalW, MAFFT, MUSCLE; if nucleotide sequences are loaded, Mauve is also provided as an option. To get the most accurate tree for your data set, please refer to our recent blog post, Two ways to find the best MegAlign Pro multiple s...
Close relatives ofpplAanddatZwere identified by BLAST searches, and aligned with Clustal Omega65(PrimPol) or the default MUSCLE algorithm in MEGA X software66(DatZ); sequence logos were made with WebLogo67. Structures homologous to PrimPol and DatZ available in PDB were identified using Dali ser...
Hsp90 is an essential molecular chaperone responsible for the folding and activation of hundreds of ‘client’ proteins, including the glucocorticoid receptor (GR). Previously, we revealed that Hsp70 and Hsp90 remodel the conformation of GR to regulate l
The alignment was carried out using Clustal Omega45 and presented using Genedoc46. Secondary structural elements of ScNsrR are indicated above the sequences. Residues that serve as cluster ligands in NsrR and IscR are indicated by boxes. Black colour indicates totally conserved residues, grey ...
Alignments and trees were constructed using Clustal Omega (EBI, Cambridge, UK) and MAFFT (Multiple Alignment using Fast Fourier Transform) [54] with default E-INS settings, and visualized using Jalview v. 2.8.2 [55] and the Molecular Evolutionary Genetics Analysis program v.5.2.2 (MEGA; ...
The variable length C-terminals past the second coiled-coil are not shown but ranged between 45 to 178 amino acids and had only 20% similarity by Clustal Omega. Uniprot also predicts that large parts of the different RIC-3 species C-terminals are intrinsically disordered, but Alphafold predicts...