Summary of the Distinguishing Test:- Nitrate Ion: Produces brown nitrogen dioxide gas when reacted with concentrated sulfuric acid and a metal.- Nitrite Ion: Does not produce a brown gas and may produce nitrogen gas instead. Show More
The resulting DNA was extracted once with an equal volume of chloroform and three times with phenol/chloroform (1:1) and precipitated with 2 volumes of EtOH at −20°C overnight. The DNA pellet was centrifuged for 15 min at 14,000 × g, washed with 70% ethanol, dried, and ...