产品名称: ACID PHENOL:CHLOROFORM 400 ML .0 订购此产品 供应商: Invitrogen 规格: EACH 目录价: 2065.5 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址: 点击查看详细 打印此页 关闭此页 上一个:现货|ACID PHENOL:CHLOROFORM 100 ML[AM9720/EACH] 下一个:PHENOL:CHLO...
chlorine['klɔ:ri:n]【氯】 chloroform['klɔ(:)rәfɔ:m]【氯仿】 Chloroformate[ˏklɔ:rә'fɔ:meit]【氯甲酸脂】 chloromethylation[klɔ:rәˏmeθi'leiʃәn【氯甲基化】 chlorosulfonation [ˏklәurәˏsʌlfә'neiʃәn]【氯磺化】 Cleavage【分离,脱除】...
This study therefore compared the performance of the conventional acid guanidinium thiocyanate-phenol-chloroform (AGPC) extraction method to QIAamp Viral RNA Mini Kit (QIAGEN, Cat. No. 52906) and OxGEn RNA Kit (OxGEn Molecular Solutions, GE-009) to build an in-house RNA extra...
Encyclopedia Wikipedia (ˌklɔːrəʊəˈsiːtɪk)or chloracetic acid n 1.(Elements & Compounds) a colourless crystalline soluble strong acid prepared by chlorinating acetic acid and used as an intermediate in the manufacture of many chemicals; monochloracetic acid. Formula: CH2...
Total RNA was extracted using phenol–chloroform extraction using TRIzol reagent (Invitrogen). Reverse transcription was performed with a First Strand cDNA synthesis kit (Roche) following the manufacturer’s instructions with random hexamers. Real-time PCR was performed with GoTaq qPCR Master Mix (Prom...
Genomic DNA extractions were carried out using the standard phenol chloroform procedure72. Genotyping PCRs were performed using GoTaq Green Master Mix (Promega, Madison, WI, USA). The oligonucleotides used in this study (Supplementary Table 9) were obtained from Integrated DNA Technologies (IDT, ...
The extracted analytes were collected on a C18 silica trap held at 20 °C and recovered after rinsing the trap with 1 ml of chloroform. Further analysis was performed by GC-MS. Under these moderate extraction conditions, a relatively clean chromatogram was obtained, with palmitic and oleic ...
Immobilization of the cells in alginate material extracted by chloroform or ethanol decreased the tolerance of the cells to phenol as compared with cells immobilized in raw alginate. This effect was diminished if the immobilized cells were exogenously supplied with palmitic acid, which is the main ...
Enzymes were deactivated by incubating samples at 90 °C for 30 min, and sample liquid (including membrane) was transferred to a 5 mL conical tube containing an equal volume of phenol:chloroform:isoamyl alcohol (P:C:I, 25:24:1, pH 7.9), which dissolved the membrane. The resulting...
30 mM DTT, 2 mM each NTP, 2 mM spermidine, 0.1 mg/mL T7 RNA polymerase, and 300 ng PCR fragments. Following DNA degradation using DNase I at the end of the transcription, the transcribed RNA products were then purified using the NaOAc/phenol/chloroform method. The purified ...