Skim milk agar plates are used to provide a nutritious medium for growing micro-organisms. Once prepared, the agar can be plated with a population of micro-organisms to test for the micro-organism's ability to digest casein protein. Casein is a large insoluble protein found in skim milk. As...
After the transformation step in a cloning protocol, bacterial cells are grown on agar plates that have an antibiotic, IPTG, and a molecule called 5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside (x-gal). The antibiotic helps select against the cells that survived transformation but did no...
The center of the enlarged cell constricts until two daughter cells are formed, each offspring receiving a complete copy of the parental genome and a division of the cytoplasm (cytokinesis). This process of cytokinesis and cell division is directed by a protein called FtsZ. FtsZ assembles into ...
coli cultures was about 108 cells/mL; 1 mL of the same culture was added to 9 mL distilled water, and several 10-fold dilutions were made and spread on agar plates. The plates were incubated at 37uC and colonies were counted after 12 h. Statistical analysis. All experiments were ...
To determine if the Sc spores are transported by both swarming and swimming motilities, we repeated the experiments with a laboratory-cultivated Bs strain that is incapable of swarming, and spore transport does not occur on the agar swimming plates (0.27% agar) (Figs. 2A and S2) [20]. ...
(EE)broth, plating on violet red bile glucose (VRBG) agar and picking of five grown colonies onto tryptone soy agar (TSA) plates, which are incubated at 25°C for 48–72 hours. Yellow-pigmented colonies, typical forC. sakazakii, on the TSA plates ar...
Agar plates used for manual ASTs are interpreted by some manufacturers as Class A tests [17]. The Clinical & Laboratory Standards Institute (CLSI) M52 and ISO 20776-2:2021 on verification of ASTs, focus on automated AST [18] and MIC-producing ASTs [19], respectively. The latter ...
It was reported that riboflavin activated by UVA showed an antimicrobial effect on agar plates inoculated with Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, and Candida albicans. The inhibition of microbial growth was significantly higher in plates treated ...
The coupling of the N-terminal building block and the global deprotection of compound 2 are described in the Supplementary Methods part 1. Thiostrepton A was purchased from Merck (Darmstadt, Germany). Agar diffusion assay To verify the neutralizing ability of AlbAS, AlbAL, AlbASM, AlbASCTD, ...
transduction technique for transferring the second plasmid into strains that contained only the first.119 Theoretically the resulting fully virulent deriva- tive strain could be selected on agar culture plates, screened for the capsule production phenotype when grown in the presence of elevated carbon ...