转染sh-lncRNA ZFAS1后,HepG2细胞中lncRNA ZFAS1表达明显降低,24 h,48 h,72 h的OD值明显减小,侵袭和迁移细胞数目明显减少,24 h的平均划痕距离明显增加(P<0.05).lncRNA ZFAS1和HMGA2在肝癌HepG2细胞中直接结合.同时转染sh-lncRNA ZFAS1和si-HMGA2后,HepG2细胞中HMGA2蛋白表达明显下降,24 h,48 h,72 ...
Bioinformatics and dual-luciferase reporter gene experiments were performed to predict and verify the targeting relationship between FAM83A-AS1 and miR-150, and miR-150 and the high-mobility family protein A2 (HMGA 2). The expression of miR-150 and ...
HMGA2凋亡目的:探讨长链非编码RNA(lncRNA)无远端同源框6反义1(DLX6-AS1)调控miR-497/高迁移率族蛋白A2(HMGA2)分子轴对骨肉瘤细胞增殖和凋亡的影响.方法:将骨肉瘤MG63细胞分为control组,si-DLX6-AS1组,si-NC组,pcDNA3.1-DLX6-AS1组,pcDNA3.1组,miR-497 mimics组,anti-miR-497组,miR-NC组,anti-miR-NC...
Objective: To investigate the effects of long non-coding RNA zinc finger antisense 1 (lncRNA ZFAS1) on the proliferation, invasion, and migration of liver cancer cells by regulating the miR-588/high mobility group AT-hook protein 2 (HMGA2) axis. Me...
CDKN2B-AS1 was validated as a sponge of miR-424-5p. Silence of CDKN2B-AS1 repressed proliferation and ECM accumulation by increasing miR-424-5p. HMGA2 was a target of miR-424-5p and miR-424-5p overexpression inhibited proliferation, ECM accumulation and PI3K/AKT pathway by targeting HMGA2...
目的:探讨长链非编码RNA锌指结构反义转录本1(lncRNA ZFAS1)调控miR-588/高迁移率蛋白A2(HMGA2)轴对肝癌HepG2细胞增殖,侵袭,迁移的影响.方法:qPCR,WB法检测80例肝癌组织及对应癌旁组织(2018年1月至2019年12月在武汉第三医院首义院区手术切除标本)及人正常肝LO2细胞和肝癌HepG2,Huh7,HCCLM3细胞中ZFAS1,miR...