Retrofitting massively parallel sequencing (MPS) for HLA-DQA1 and polymarker (PM) in forensic caseworkForensic genotypingAllele-specific oligonucleotide hybridizationAmpliType forensic DNA amplification and typing kitMassively parallel sequencingParalogous genes...
Espinheira R, Ribeiro T, Geada H, Reys L (1994) Polymarker and HLA DQA1 genetic markers in forensic casework. In: Mangin P, Ludes B (eds) Acta Medicinae Legalis, Springer, Berlin Heidelberg New York, pp 37–38Espinheira R, Ribeiro T, Geada H, Reys L (1994) Polymarker and HLA DQA1...
可 见较 清晰 DNA 条带 ,与标准 DNA 分子量 Marker ( 100bp.600bp) 比较 带型的位置 ,确定基 因型,如图 1 示。(5) 质量 的控制 :假阳性控制 :经 PCR反应后扩增 的基因片段数是一定的且亮度较强 。假阴性控制 :每 个 反应管均加入 内参引物(在 l 3 个泳道 内均出现扩 ...
物技术 有限公 司) ;(5) DL2000 DNAmarker( 大连 宝生 物 工程有 限公 司 ) 。 1 .4 PCR.SSP 法检测 HLA.DQ A1 基因型 试剂盒 提取法 提取基因组 DNA ( 严格按试剂盒说 明书 操 作)后 ,序列特异性引物.聚合 酶链 反应 ( Polymerase ...
Abstract: The HLA DQB1*0302 allele on DR4 haplotypes is a well-known marker for T1D susceptibility. The contribution of this DQ molecule to overall disease... Y Park,BD Tait,E Kawasaki,... - 《Annals of the New York Academy of Sciences》 被引量: 11发表: 2004年 HLA-DQ allele competi...
HLA DQA1 and Polymarker validations for forensic casework: standard specimens, reproducibility, and mixed specimens. This study describes the testing performed by the Minnesota Forensic Science Laboratory (MFSL) to validate the Amplitype DQA1 and Amplitype Polymarker (PM)... AM Gross,RA Guerrieri -...
The B*44:27:01 allele, an east European marker considered a rare variant according to the “Rare Alleles Detector” tool9, was also noticed in our cohort at a relatively high frequency (2.7%), contrasting observations from Croatian HSCT patients (1.18%)19, Czech National Marrow Donors ...
Although we did not sequence exon 1, we were able to distinguish both alleles by a surrogate marker in intron 2, thus clearly identifying DQA1*03:03. Klitz et al. did not investigate polymorphisms outside exon 2 and was, therefore, not capable of identifying the DQA1*03:03 allele [14]....
多标记系统(polymarker,PM)位点检测技术包括有低密度脂蛋白的受体(LDLR),血型糖蛋白A(GYPA),血红蛋白G球蛋白(HBGG),型特异性成分(GC)及D7S8等5个位点,分别位于第19,4,11,4,7号染色体上.HLADQA1位点位于HLAⅡ类基因HLA-DQ亚区中的DQA1基因座,在其第三个外显子中存在一个等位基因变异区,该区经克隆和...
Examines the HLA-DQA1 and polymarker in the Uros population from Peruvian Highland. Native language of the Uros population; Ethnic characteristics; DNA typing.Journal of Forensic Sciences (Wiley-Blackwell)TorresLaboratorio de Biotechnología GenéticaGladys...