There have been no prior means of excluding this condition without surgical exploration and histopathologic study of the excised tissue. A genetic predisposition is suggested for the development of idiopathic primary retroperitoneal fibrosis in patients who are HLA-B27 antigen positive. In this study ...
Twenty of these were HLA-positive and 9 B27 negative. Thirty matched controls were also studied and of these, 15 were negative. Random and directed were measured both by migration through a micropore filter and also by migration under an agar film. The attractant was serum activated either by...
Failure of the serological determination of HLA-B27 due to antigen masking in patients with ankylosing spondylitis In patients suffering from ankylosing spondylitis (AS) HLA-B27 determination by means of the microlymphocytotoxicity test (MLCT) sometimes gives equivocal ... J Neumüller,M Fischer,R ...
the surface of peripheral blood lymphocytes (PBL) by means of an anti-HLA-B27 allospecific monoclonal antibody 2-dimensional gel electrophoresis was used to compare the structure of the B27 antigens derived from 5 patients with ankylosing spondylitis with that of healthy HLA-B27 positive ...
Previous investigations showed an increased antibody response to the Klebsiella capsular types K26, K36, and K50 in sera from HLA-B27-positive AS patients. In the present study the prevalence and titers of antibodies against Klebsiella capsular antigens were measured by means of an ELISA in 32 ...
Antigen maskingIn patients suffering from ankylosing spondylitis (AS) HLA-B27 determination by means of the microlymphocytotoxicity test (MLCT) sometimes gives equivocal or false-negative results even though it has been performed with meticulous care. These failures of the test did not arise when ...
In the present study the prevalence and liters of antibodies against Klebsiella capsular antigens were measured by means of an ELISA in 32 sera from HLA-B27-positive AAU patients either with (n = 10) or without AS (n = 22) and compared with sera from HLA-B27-negative AS-patients (n ...
2.2. HLA-B27 Determination Flow cytometry and/or PCR tests were used to determine HLA-B27 positive. Whole blood samples were analyzed by flow cytometry for HLA-B27 using the BDTM HLA-B27 kit (BD Biosciences, Franklin Lakes, NJ, USA), which combines fluorescein-conjugated anti-HLA-B27 and ...