METHODS: We examined the following patients for MICA gene polymorphism by means of polymerase chain reaction and subsequent automated fragment detection by fluorescent-based technology: 64 (37 HLA-B27-positive and 27 HLA-B27-negative) whites with acute anterior uveitis, 74 (67 HLA-B27-negative and...
A marked positive expression was observed for HLA-A, HLA-B/C or B2M in a third of lesions, which is less than seen in cutaneous melanoma (Campoli and Ferrone, 2008). The level of expression of HLA Class I in CoM was not related to the tumour's basal diameter, development of ...
Zheng G, Guo Z, Li W, Xi W, Zuo B, Zhang R, et al. Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab. Signal Transduct Target Ther. 2021;6(1):236. Article CAS PubMed PubMed Central Google Scholar Fons P, Chabo...
Generation of human iPSCs from cells of fibroblastic and epithelial origin by means of the oriP/EBNA-1 episomal reprogramming system. Stem Cell Res Ther. 2015;6:1–7. Article CAS Google Scholar Sullivan S, Fairchild PJ, Marsh SG, Müller CR, Turner ML, Song J, Turner D. Haplobanking...
CMV-positive patients were treated with either ganciclovir or foscarnet. Evaluation of engraftment and transplantation-related toxicity Neutrophil engraftment was defined as an absolute neutrophil count (ANC) ≥ 0.5 × 109/L for 3 consecutive days. Platelet (PLT) engraftment was defined as ...
[9]. Due to the promising potential of HLA-G blockade, ongoing researches about why and how HLA-G-based therapeutic approaches quell the malignant phenotype are increasing, as exemplified by the deciphering of the roles of HLA-G positive cancer cells in colorectal cancer using spatial and ...
01 with and without exchange peptide was included on each plate as positive and negative controls for exchange, respectively. The well-characterized A*02:01-specific viral epitope, CMV pp65 peptide (NLVPMVATV, Elim Biopharmaceuticals), was plated in quadruplicate as a positive control for peptide ...
A few TCR variants that recognize these DQ/antigen complexes are on CD4 positive (CD4+) T-cells. These T-cells, called T-helper cells, can promote the amplification of B-cells which, in turn recognize a different portion of the same antigen. Alternatively, macrophages and other megalocytes...
Thus, in the present study we undertook a systematic and comprehensive analysis of the effects of AS-associated ERAP1 polymorphism on the HLA-B*27 peptidome. We compared the B*27:05 peptidomes from ERAP2-positive cells expressing distinct ERAP1 haplotypes by means of a homogeneous and high-...
Briefly, genomic DNA was amplified by means of PCR with primers specific for the HLA-A, HLA-B, HLA-C, DRB1, and DQB1 loci. This amplification provides a sequencing template for the exons required to genotype at allele-level resolution. The assignment of HLA type is based on comparison ...