温州医学院硕士论文 印病毒LMP2HLA-A*0201限制性CTL表位预测、筛选及鉴定 中文摘要 目的: ・ 1.从SwissProt蛋白数据库获得EB病毒(EBV)潜伏膜蛋白2(LMP2)的全长氨摹酸 序列,利用争物信息软件对EBVLMP2中的HLA—A*0201限制性CD8+细胞毒特异T细胞 (cytotoxicity Tcell,CTL)表位进行预测。 2.筛选 以SYFPEITHI预...
sequencewasconfirmedbyDNAsequenceanalysis.The recombinantplasmidpBV220-HLA-A*0201-BSPwastransformedto thecompetentcellsofDH5α.TheresultsshowedthattheHLA- A*0201-BSPfusionproteinwassuccessfullyexpressedintheformof inclusionbodyandamountedtoover28%oftotalcellproteinsvia inductionat42℃.AfterwashedwithtritonX-100...
.乙肝病毒x 蛋白H L A —A 0 20 1限制性C T L 表位的筛选与鉴定朱焱, 李卡, 郭瀛军, 剥、 树汉+ ( 第二军医大学基础部医学遗传学教研室, 上海200433)・论著・[ 摘要]目 的: 应用T 2细胞结合实验毗及亲和力稳定性实验鉴定由网络表位预测服务和通用原则预测得到的乙肝病毒x 蛋白( H B x )来寐...
Using a suited algorithm, the amino-acid sequence of prostein was screened for peptides predicted to bind to HLA-A*0201, the most common HLA class I allele in Caucasian individuals (Table 3). Peptides that effectively bind to HLA-A*0201 are usually nonamers or decamers with the typical ...
EXPERIMENTAL DESIGN: In our study, a HLA-A2.1-restricted CTL epitope was identified by using the following four-step procedure: (a) computer-based epitope prediction from the amino acid sequence of TRAG-3 antigen; (b) peptide-binding assay to determine the affinity of the predicted peptide ...
(C terminus). Second, the sequence of the candidate epitope should be highly conserved in most D1V strains. If a candidate epitope has a high affinity for HLA-A*0201 as confirmed by an MHC peptide complex stabilization assay, its variant peptides in D2V (NGC strain; AAC59275), D3V (...
The amino acid sequence of the precursor is indicated at the top and the individual amino acid substitutions at position 278 (X = Q, X = S, and X = H) in the upper right corner of each spectrum. The spectrum corresponding to the digestion of the peptide analog His278 in the presence...
These data suggested either that each CTL clone recognized a different modified form of this peptide or that the true epitope for either or both CTLs was a naturally processed peptide with a primary sequence different from FIDSYICQV. The most likely site for modification of FIDSYICQV is the ...
By identifying two distinct epitopes within MSH3(-1), the sustained immunogenicity of the frameshift mutated sequence was confirmed. Our data therefore encourage further exploitation of MSH3 as a piece for peptide-based vaccines either for therapeutic or –even more important– preventive purposes. ...
(LMP2) Firstlv,amino protein sequences SYFPEITHI from database.Secondly,database wereobtained SwissProt.protein 1restricted and were to HLA—A宰020 NetCTL、HLA.Bind appliedpredict Rankpep EBVLMP2CTL afeselected inEBV epitopes CTL LMP2,then epitopes possible comprehensively. ofCTL 2.Synthesisepitopes ...