PCR/SSP(Sequencespecificprimer,序列特异性引物)是一种新的基因多态性分析技术,可用于HLA复合体及其他具有多态性特点的基因分型.我室于1993年建立这种方法,并对正常... 熊平,杨颖,龚非力 - 《免疫学杂志》 被引量: 86发表: 1996年 The Association Between HLA-A Alleles and an Alu Dimorphism Near HLA-G ...
PCR/SSP方法用乃设计出一整套等位基因组特异性引物（sequence specific primer,SSP），借助PCR技术获得HLA型别特异的扩增产物，可通过电泳直接分析带型决定HLA型别，从而大大简化了实验步骤。优点是简单易行, 分辨率可从低到高, 成本低 。缺点是不易自动化;不能检测新的等位基因，试剂盒需不断升级。此方法适宜零散...
Bettinotti MP, Hadzikadic L, Ruppe E, Dhillon G, Stroncek DS, Marincola FM: New HLA-A, -B, and -C locus-specific primers for PCR amplification from cDNA: application in clinical immunology. J Immunol Methods 2003, 279 : 143-148. PubMed Abstract Publisher Full Text Return to...
目前实验室HLA分型大多为基因分型方法,主要包括低分辨水平的PCR-序列特异性引物基因扩增法(sequence specific primer, SSP)、中分辨水平的PCR-序列特异性寡核苷酸探针法(sequence-specific oligonucleotide, SSO)、基因芯片和高分辨水平的PCR-直接测序分型(sequence-based typing, SBT...
A:基因名称 *:分隔符 02:等位基因组,常指血清学特异性 :字段分隔符 101:特定HLA蛋白质 01:编码基因内显示同义的DNA替换 02:非编码区与的差异 N:后缀,用来表示基因表达的变化 HLA 基因分型检测方法 (1)聚合酶链反应-序列特异性引物(polymerase chain reaction-sequence specific primer, PCR-SSP)方法 ...
With the development of polymerase chain reaction (PCR), DNA-based techniques have dominated in typing laboratories due to their precision and reproducibility as well as enhanced sensitivity and resolution. DNA-based techniques include PCR-sequence specific primers (SSP), PCR-sequence-specific oligonucle...
PROBLEM TO BE SOLVED: To obtain the subject new primers having specific nucleic acid sequences, consisting of the primers used for distinguishing HLA-DQA1 allele, being discriminating markers for recognizing self with non-self and also useful for performing the typing of the above gene easily.收...
目前世界卫生组织推荐的HLA分型方法的“金标准”是PCR-SBT(polymerase chain reaction-sequence specific primer),检测座位覆盖HLA-A、-B、-C、-DRB1和-DQB1,广泛应用于临床移植供受者HLA基因分型。然而由于HLA基因高度多态性和检测技术的限制,在检测特定座位的分型中存在模棱...
HLA复合体的遗传特点 1复等位基因2共显性遗传3极高的多态性 截至2009年10月 HLAI类基因-抗原结构特点 HLAII类基因-抗原结构特点 2HLA分型技术 血清学水平 基因水平 SSP (sequencespecificprimer)SSO (sequencespecificoligonucleotide)SBT (sequence-basedtyping)HLA测序分型（SBT）的PCR策略 HLA–A、C HLA–B HLA...