Keywords:HL一60cell;Differentiation;Cellsurfacemarkers;Streptococcuspneum o nia;Opsonophagocytickillingassay (OPKA) 肺炎链球菌(Streptococcuspneumonia,Sp)主要 引起人类常见的肺炎、脑膜炎、中耳炎和败血症等疾 病。全世界有5%一10%健康成人和20%一40%健 ...
Morphological analysis, flow-cytometric determination of granulocytic or monocytic surface markers, and ability to reduce nitroblue tetrazolium (NBT) demonstrated that expression of exogenous wild-type p53 gene in HL-60 cells induces differentiation through the granulocytic pathway. Proliferation and cell-...
KaryotypeThe stemline chromosome number is pseudodiploid with the 2S component occurring at 6.2%. Five markers (M2 through M6) were common to most S metaphases. DM's, which varied in numbers per cell, occurred in all metaphases karyotyped. HSR chromosomes were not detected. ...
We have produced a murine IgM monoclonal antibody (Y201) that recognizes a cell surface antigen present on HL60 cells. Seventy percent of uninduced HL60 cells expressed Y201 antigen, while the remainder did not. There were no morphological differences between HL60 cells that expressed Y201 anti...
Table 1. HL-60 Cell Differentiation TreatmentCell Adhesion*NSE† StainingCell Surface Markers‡ MolMy4 Untreated 4.0 ± 2.1 + 4.5 ± 1.4 13.4 ± 5.9 Bryostatin 1,10 nmol/L 27.3 ± 5.5 + + + 22.0 ± 9.0 49.0 ± 4.5 Bryostatin 1. 100 nmol/L 8.7 ± 4.3 + + 19.6 ± 7.8 45.3 ±...
The expression of a nonspecific cross-reacting antigen (NCA) species on the cell surface of the human promyelocytic leukemia cell line HL-60 was investigated via binding of I-labeled carcinoembryonic antigen (CEA) and NCA-specific monoclonal antibodies (Mabs). Very low specific binding of the CEA...
[phorbol 12-myristate 13-acetate (PMA)], they shift from suspension to adherent on the surface of the tissue culture vessel, assume the appearance of macrophages, and acquire macrophage-associated surface markers, the enzyme-nonspecific esterase, the isozyme acid phosphatase, and the myeloid-...
Treatment of two human leukemia cell lines with 1.25% dimethyl sulfoxide at 37 °C results in a rapid increase in the number of transferrin receptors on the cell surface detected by fluoroscein-labeled anti-transferrin receptor antibodies. Both HL-60 cells, a human myeloid cell line, and K562...
CD38 expression increased, the proportion of cells in G1/G0increased, and cell population growth was retarded compared to control cells not treated with RA. Addition of F-araNAD+or dF-araNAD+at either time point, however, had no effect on the induced expression of any of these markers. ...
The binding of 125I-labeled human hemopexin to human leukemia HL60 cell at 4 degrees C was saturable with time and with increasing concentrations of 125I-hemopexin. Scatchard analysis of the binding data revealed the presence of approximately 42,000 binding sites/cell with an apparent dissociation...