2.4. Cell culture Cardiac muscle cell line, designated as HL-1 cells were derived in the Claycomb laboratory from the AT-1 mouse atrial cardiomyocyte tumor lineage [35], [36], [37]. Non-beating HL-1 cells (NB HL-1) were obtained from the HL-1 line developed by W. Claycomb by growi...
Uterine serous carcinoma (USC) is the most aggressive form of endometrial cancer, with poor survival rates and high recurrence risk. Therefore, the purpose of this study was to identify therapeutic targets that could aid in the management of USC. By anal
(Refer to Protocol B: One step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.004 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should ...
Cell culture media were replaced every 2 days until mature osteoclasts had formed. After 10 days, the osteoclasts were removed from the surface using hypochlorous acid. The percentage of resorbed bone surface area was quantified using Image J software. Western blot Western blot analysis was ...
(All from Life Technologies GmbH). A single-cell suspension of bone marrow cells was seeded in 100 mm culture dish, non-adherent cells were removed after 24 h, and medium was replenished every 2 days. Cells were used at the 9-16th passages. Human bone marrow MSCs were purchased ...
S-nitrosylation of UCHL1 induces Parkinson's disease phenotype in mouse model. Our in vitro and cell culture based studies demonstrated that S-nitrosylation of UCHL1 hindered its cellular function through destabilization of native structure and subsequent cellular aggregation. That may be one of...
(RSEM) protocol using in-built scripts in the Trinity software package [61]. The normalization of the data among different libraries was performed using the Trimmed Mean of M-values normalization method in Trinity. The obtained count value was exported to Bioconductor software package DESeq2 [62...
Live cell imaging of PH-AKT-GFP MEFs were plated at 1 × 105 cells per well in 6-well tissue culture plates with glass coverslips 48 h before imaging. At 24 h, cells were transfected with PH-Akt-GFP. On the day of experimentation, cells were serum starved for 3 h, coverslips were ...
(Refer to Protocol B: One step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.004 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be ...
(Refer to Protocol B: One step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.004 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined ...