Antibody 克隆号 8B8P5 抗原 A synthetic phosphorylated peptide around T3 of human Histone H3 (P84243) 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 0.5 mg/mL 纯化类型 Affinity Chromatography 保存液 PBS, pH 7.3, with 50% glycerol, 0.05% BSA 内含物 ...
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, ...
. Then 2 μg anti-EZH2 antibody (Cell Signaling Technology, Cat.#5246) was added to the chromatin in dilution of 1:100 and incubated on a rocker overnight at 4 °C. Protein A/G-magnetic beads (30 µl MCE, Cat.#HY-K0202) were added for 3 h incubation. The beads were...
Technically, we found the evaluation of HDAC2 immunostainings rather straightforward, as the percental nuclear immunoreactivity was relatively independent of varying antibody concentrations. This is a prerequisite for a robust semiquantitative evaluation and is likely to reduce interlab variability and makes...
该通用组蛋白H3-K4甲基化分析试剂盒 内主要产品组份包括:GD1 (10x lysis buffer) GD2 (Extraction buffer) GD3 (10x wash buffer) GD4 (Histone buffer) GD5 (Blocking buffer) GD6 (Antibody buffer) GD7 (capture antibody, 100 ug/ml) GD8 (detection antibody, 400 ug/ml) GD9 (developing solution...
In vitro anti-histone antibody produc- tion by peripheral blood cells from patients with systemic lupus erythematosus. Clin lmmunol lmmunopathol 1988; 47: 343-53.O'Dell, J.R., Bizar-Schneebaum, A., Kotzin, B.L., 1988. In vitro anti- histone antibody production by peripheral blood cells...
重庆市华雅干细胞技术有限公司>>抗体/抗原>> ab9050Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) 在线沟通: 重庆市华雅干细胞技术有限公司 颜先生(经理) 电话: 400-021-2200 手机: 15021010459 传真: 023-63419626 联系我时, ...
33. A monoclonal antibody which recognizes histone H1 or a histone H1-like antigen present in the cell membrane of a spleen cell, wherein the monoclonal antibody specifically recognizes an amino acid sequence represented by SEQ ID NO: 8. 34. The monoclonal antibody according to claim 33, whi...
After centrifugation at 12,000×gfor 10 min at 4 °C, 12 μL of the supernatant was taken out for input. The left supernatant was transferred to the 200 μL tube containing 110 μL suspended antibody-coated Protein A beads, then incubated on a tube rotator overnight at 4 ...
(CD4+T-cells versus non-CD4 cells) suggested to us that the various cell types might be affected to different extents by TSA. To clarify this point, we cultured PBMCs in the presence of either soluble anti-CD3 antibody (+CD3) or IL-2 (+IL-2) and treated with TSA (+TSA) or DMSO...