An aliquot of the oligonucleotide solution (3 ml) was mixed with 50 ml of MilliQ-purified water (milliQ water), transferred into an electroporation cuvette (1 mm gap), and electroporated using a BIO-RAD Micro Pulser. When the time constant was ,5.5 milliseconds, another aliquot of the ...
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In order to generate multi-gene expression cassettes, the baculovirus expression vector pACEBac1 was modified by adding two restriction sites for the type IIS enzyme BsaI; one upstream of the promoter, and one downstream of the terminator. Cleavage of the resulting vector with the BsaI enzyme the...
b M protein was immunoprecipitated by NF45 and PCNA translation. The GO annotations of the host cellular pro- teins that interacted with M protein indicated they were located on the membrane and had binding and catalytic activities, and we inferred that M protein could combine with membrane ...
025. Thismeant that one out of four unemployed people had graduated from a HEI.The highest unemployment rates among graduates were concentrated inthose disciplines where educational enrolment had been particularly con-centrated in previous years. In 2001, economics graduates were the largestunemployed ...
To start, the president-elect fired off a series of tweets around 6:30 a.m. E.T. calling Streep “one of the most over-rated actresses in Hollywood” and a “Hillary flunky who lost big.” During her emotional acceptance speech for the Cecil B. DeMille Award for lifetime achie...