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An aliquot of the oligonucleotide solution (3 ml) was mixed with 50 ml of MilliQ-purified water (milliQ water), transferred into an electroporation cuvette (1 mm gap), and electroporated using a BIO-RAD Micro Pulser. When the time constant was ,5.5 milliseconds, another aliquot of the ...
Construction of mutants of promoter Pylb by site-directed mutagenesis. The above results demon- strated that the shortest functional sequence of Pylb was F4 and revealed its putative recognition element (− 35 box and − 10 box). To experimentally analyze the cis-acting elements of...
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For example, an acetamidino group is incor- porated in the flagellum N-glycan of Methanococcus mari- paludis [33], while formyl and amino substitutions have been observed on sugars in the flagellin glycan of Pseudo- monas aeruginosa [14]. An acetyltransferase encoded in the flagellar region ...
Peptides were sep- arated using a nano-flow HPLC (LTQ VELOS, Thermo Finnigan, San Jose, CA, USA). Bioinformatics analysis The functional annotation and classification of all the proteins were determined using Blast2GO program [40] against the non-redundant protein database (nr) at NCBI and...
The cells were electroporated with a Gene Pulser (Bio-Rad) using 250 microfarads at 450 V and then resuspended in 30 ml of growth medium and plated on 9-cm dishes and glass coverslips for Western blot analysis and immunostaining, respectively. Calcium Phosphate-mediated Transfection Breast ...
Rapid degeneration of recently formed Y chromosomes has been demon- strated in Drosophila (Bachtrog et al., 2008) and sticklebacks (Peichel et al., 2001). However, there are few empirical data for W chromo- somes. The data presented here suggest that the W chromosomes of Triportheus have...
The percentage of expression of RQR8 on the surface of electroporated T cells was similar when using the minRNA design and an optimized (based on SpikeVax®) design (Figure 5A), although the signal intensity was slightly higher with minRNA. As a result, target cell killing was similar ...
Since 9V from a DC supply was used for take-off with wires, the input power exceeded the motor-rated power. Based on the rated current, at least 18.24 W was needed for the 22.7 g model to take off, resulting in a power efficiency of 0.012 N/W. Compared to this, the power ...