Enhanced clearance of highly protein-bound drugs by albumin-supplemented dialysate during modeled continuous hemodialysis. Nephrol Dial Transpl. 2009;24(1):231-8.Churchwell MD, Pasko DA, Smoyer WE, Mueller BA. Enhanced clearance of highly protein-bound drugs by albumin-supplemented dialysate during ...
Solid phase extraction (SPE) in some cases yields poor recovery of highly protein bound drugs, and this phenomenon has been explained by insufficient retention of the analyte on the sorbent [1]. The problem may be surmounted by changing the pH of the sample, by addition of protein denaturing...
Therefore, there is a need for a new integrin therapeutic modality with (i) high selectivity for a single RGD integrin heterodimer, (ii) atomic-level control over the precise location of the target binding site and the protein-protein interaction interfaces to control the evoked integrin ...
The dialyzed cell supernatant was applied to Nickel-NTA agarose beads (Qiagen, Hilden, Germany) using a gravity-flow column (BioRad, Hercules, CA, USA), washed with 20 column volumes of IMAC wash buffer (20 mM Tris, 500 mM NaCl, 30 mM Imidazole, pH 8.0) and the bound protein was ...
Especially, the KB,vivo for the highly bound drug S(−)-propranolol on the basis of total drug (13 ± 4.7 nM) deviated significantly from the KB,vitro (1.9 ± 0.48 nM).25–28 Whereas on the basis of free drug concentration the KB,vivo (2.0 ± 0.71 nM) closely resembled the ...
Fig. 2. The effect of morphine analogues on G-protein activity compared to the parent ligands in [35S]GTPγS binding assays in rat brain membrane homogenates. Figure legend: “Total” on the x-axis indicates the basal activity of the monitored G-protein, which is measured in the absence ...
(paclitaxel protein-bound); Celgene), displaying modest activity against multiple cancer cell types (i.e., IC50s in the tens to hundreds of nM range)11,12. Additionally, they have generally relied on drug encapsulation as opposed to chemical conjugation; as a result, these NPs have displayed...
Structural patterns common to GPI-AP proteins enable computer algorithms to identify GPI-AP candidates by analyzing protein primary structure. Several algorithms have been developed using well-characterized GPI proteins as a training set. Big-PI [41, 42] and DGPI [43] were designed to detect spec...
(Standard Diagnostics, Suwon city, Republic of Korea) for detecting the histidine-rich protein 2 (HRP2) ofP. falciparumaccording to the Manufacturer’s instructions. Then, 70 µl of blood were sampled with a single-use 75 mm micro-hematocrit capillary tube (Thermo Fisher Scientific, MA, USA...
Since the protein is a flexible molecule, its carbohydrate-bound (holo) conformation could be different from the carbohydrate-free (apo) one. We employed the experimental holo structure dataset T59 and the predicted apo structures dataset T59AF2to assess all methods for carbohydrate-binding site pr...