Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Dropletsdoi:10.1016/j.cell.2015.05.002Summary Cells, the basic units of biological structure and function, vary broadly in type and state. Single-cell genomics can characterize cell identity and function, but ...
Z. et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell 161, 1202–1214 (2015). Article CAS Google Scholar Han, X. et al. Mapping the mouse cell atlas by Microwell-Seq. Cell 173, 1091–1107.e17 (2018). Article Google Scholar Gie...
Z. et al. Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell 161, 1202–1214 (2015). Article CAS PubMed PubMed Central Google Scholar Theberge, A. B., Whyte, G. & Huck, W. T. S. Generation of picoliter droplets with defined contents ...
Conclusions In this study, RNA-Seq and differential expression anal- ysis in jojoba male and female flowers was conducted for the first time. The genes in the sex specific parts of the genome may be expressed differentially in other tis- sues but the flowers of the male and female are the...
Platelet transfusion therapy has made a great breakthrough in clinical practice, and the differentiation of human embryonic stem cells (hESCs) to produce functional platelets has become a new potential approach, however, efficient generation of functiona
A new method to customize protein expression vectors for fast, efficient and background free parallel cloning BMC Biotechnol., 13 (2013), Article 12 View in ScopusGoogle Scholar 177 P.T. Lowary, J. Widom New DNA sequence rules for high affinity binding to histone octamer and sequence-directed...
Through a massive parallel sequencing of the 5’ end of cDNA and analysis of the sequenced tags, transcription start sites and transcripts amount are inferred on a genome-wide scale. Thus, CAGE provides an effective genome-wide transcriptional profiling as an alternative to microarray and RNA-seq...
During a genome-wide screen with RNA-mediated interference, we isolated CG8580 as a gene involved in the innate immune response of Drosophila melanogaster. CG8580, which we called Akirin, encoded a protein that acted in parallel with the NF-kappaB transcription factor downstream of the Imd pathwa...
Highly parallel lab evolution reveals that epistasis can curb the evolution of antibiotic resistance Abstract Genetic perturbations that affect bacterial resistance to antibiotics have been characterized genome-wide, but how do such perturbations interact with subsequent evolutionary adaptation to the drug?
The rapid and highly parallel identification of antibodies with defined biological activities by SLISY Steve Lu, Austin K. Mattox, P. Aitana Azurmendi, Ilias Christodoulou, Katharine M. Wright, Maria Popoli, Zan Chen, Surojit Sur, Yana Li, Challice L. Bonifant, Chetan Bettegowda...