The expression of anti-apoptotic molecule Bcl-2 in neonatal granulocytes was also lower than that in adult granulo- cytes (4.64 Ϯ 0.51 versus 7.24 Ϯ 1.17 unit/mg protein, p ϭ 0.032). Moreover, another anti-apoptotic signal, nuclear fac- tor-B nuclear translocation, was also ...
Controls were as described for the samples with the following differences; one slide was prepared with primary antibodies and no secondary antibodies to check the background signal, and another with secondary antibodies and no primary antibodies, to determine the level of non-specific binding of the...